Urea → L-Tryptophan

HRID 476589

Reaktionsgleichung

Reaktionsgleichung von HRID 476589
[CH:1]1[C:6]([C@@H:7](O)[C@H:8]([NH:11]C(C(Cl)Cl)=O)CO)=[CH:5][CH:4]=[C:3]([N+]([O-])=O)[CH:2]=1.O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.S([O-])([O-])(=O)=O.[NH4+].[NH4+].[NH2:40]C(N)=O.O.O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Mg+2].CC1[N+](CC2C=NC(C)=NC=2N)=CSC=1CCO.Cl.[Cl-].[OH:77][C:78]([CH2:80][CH2:81]CC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:79]>N[C@H](C(O)=O)[C@@H](C)O>[NH2:40][C@H:80]([C:78]([OH:77])=[O:79])[CH2:81][C:7]1[C:6]2[C:5](=[CH:4][CH:3]=[CH:2][CH:1]=2)[NH:11][CH:8]=1

Komponenten

9

Edukt 7

HCID 1176UreaHCID 5959Chloramphenicol2×HCID 6202Thiamine HydrochlorideHCID 24843Magnesium Sulfate HeptahydrateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 6097028Ammonium Sulfate

Produkt 1

HCID 6305L-Tryptophan

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 6288L-ThreonineLösungsmittel2×

Versuchsvorschrift

After culturing at 30° C. for 2 weeks, about 10,000 colonies resistant to chloramphenicol appeared, which were replicated in threonine-free medium (Thr deficient medium) which contained 2% of glucose, 1% of ammonium sulfate, 0.25% of urea, 0.1% of dihydrogen potassium phosphate, 0.04% of magnesium sulfate heptahydrate, 1 mg/dl of FeSO4.7H2O, 1 mg/dl of MnSO4.4H2O, 200 μg/dl of thiamine hydrochloride and 50 μg/dl of biotin, pH 7.0, 1.8% of agar, and 3 strains which were resistant to chloramphenicol and tryptophan independent were obtained.

Quellen und Nachweise

ORD-Eintrag
ord-ec4b76b4beb443f0b3ee53df3e72b8aa
Quelldatensatz
uspto-grants-1989_12
Patentnummer
US04885245

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