Ethanol → Ninhydrin

HRID 485081Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 485081
C1N[C@@H](C(O)=O)CN([CH2:10][CH2:11][CH2:12][C:13]([OH:15])=[O:14])C1.[CH3:16][CH2:17][OH:18].[CH2:19](O)[CH2:20][CH2:21]C.C(O)(=[O:26])C.O>>[CH:19]1[CH:10]=[C:11]2[C:12]([C:13]([OH:14])([OH:15])[C:17](=[O:18])[C:16]2=[CH:21][CH:20]=1)=[O:26]

Komponenten

4

Edukt 3

HCID 702EthanolHCID 22056973Butanol acetic acid waterHCID 44277691HCID 44277691, Verbindung

Produkt 1

HCID 10236Ninhydrin

Versuchsvorschrift

The column is washed with 80 l of water, followed by passage of 240 l of 0-10% EtOH at SV=1.0 (80 l/hr.). The eluate is collected in 8 l fractions which are assayed by HPLC. The HPLC gives an elution curve corresponding to the first half of the attached drawing. It is clear that D-CPC and DA-CPC are eluted in two distinct patterns. A further amount of 10% EtOH may be passed to elute CPC but in order to save on the elution time and obtain a better-defined elution curve, the eluant is replaced with 20% EtOH and the eluate is collected in 8 l fractions. As assayed by HPLC, there is obtained an elution curve corresponding to the second half of the drawing. Each fraction is applied to a thin-layer chromatoplate of microcrystalline cellulose powder (Tokyo Kasei K.K. Japan) and the chromatogram is developed with n-butanol-acetic acid-water (3:1:1, V/V), and dried in air. The image formed by color-reaction with ninhydrin shows a spot each at Rf=0.15 for D-CPC, RF=0.28 for CPC and Rf=0.30 for DA-CPC.

Aufarbeitung

  1. washThe column is washed with 80 l of water
  2. customThe eluate is collected in 8 l fractions which
  3. customThe HPLC gives an elution curve corresponding to the first half of the attached drawing
  4. washare eluted in two distinct patterns
  5. washto elute CPC
  6. customobtain a better-defined elution curve
  7. customthe eluate is collected in 8 l fractions
  8. customthere is obtained an elution curve corresponding to the second half of the drawing
  9. customdried in air

Quellen und Nachweise

ORD-Eintrag
ord-4573794d7611458084fec677ad581975
Quelldatensatz
uspto-grants-1985_08
Patentnummer
US04535155

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