L-Leucine → L-Tyrosine

HRID 495333

Reaktionsgleichung

Reaktionsgleichung von HRID 495333
[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH2:3][CH:4]([CH3:6])[CH3:5].C(O[C:13](=[O:20])[C@H:14](CC(C)C)N)C.[NH2:21][C@H:22]([C:27]([OH:29])=[O:28])[CH2:23][CH2:24][S:25][CH3:26]>>[NH2:21][C@H:22]([C:27]([OH:29])=[O:28])[CH2:23][CH2:24][S:25][CH3:26].[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH2:3][C:4]1[CH:6]=[CH:14][C:13]([OH:20])=[CH:22][CH:5]=1

Komponenten

5

Edukt 3

HCID 6106L-Leucine2×HCID 6137L-MethionineHCID 75971L-Leucine ethyl ester

Produkt 2

HCID 6057L-TyrosineHCID 6137L-Methionine

Versuchsvorschrift

The papain-catalyzed synthesis of leucine and HMB co-oligomers was performed. Synthesis of leucine oligomers and HMB-leucine co-oligomers was initiated with leucine ethyl ester and HMB ethyl ester as the substrates. The overall synthesis and purification approach was similar to the one used in the case of methionine and HMB-methionine. Reaction rates similar to those obtained with methionine and tyrosine were achieved. Approximate oligomer yield was 58%. The freeze-dried oligomer precipitates were solubilized in DMSO. The solution concentration was brought to approximately 2 μg/μl The solution was mixed with 1:1 acetonitrile:water mixture containing 0.1% acetic acid. The total fluid volume entering the ESI-MS was maintained at 0.2 mL min.−1 The positive mass spectrum of the leucine oligomers is shown in FIG. 33A.

Aufarbeitung

  1. customThe overall synthesis and purification approach
  2. customReaction rates similar to

Quellen und Nachweise

ORD-Eintrag
ord-dc7beefece054ece995580c4f7aab15d
Quelldatensatz
uspto-grants-2005_09
Patentnummer
US06939693B2

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