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Dimethylformamide → 8-(6-(4-tert-butylphenyl)benzo[d][1,3]-dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H-purin-6-amine

HRID 521581
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Druckvorschau

Reaktionsgleichung

Reaktionsgleichung von HRID 521581
[C:1]([C:5]1[CH:10]=[CH:9][C:8](B(O)O)=[CH:7][CH:6]=1)([CH3:4])([CH3:3])[CH3:2].[CH3:14][CH:15]([NH:17][CH2:18][CH2:19][CH2:20][N:21]1[C:30]([S:31][C:32]2[CH:37]=[C:36]3[O:38][CH2:39][O:40][C:35]3=[CH:34][C:33]=2I)=[N:29][C:23]2[C:24]([NH2:28])=[N:25][CH:26]=[N:27][C:22]1=2)[CH3:16].C([O-])(O)=O.[Na+].CN(C=O)C>Cl[Pd](Cl)([P](C1C=CC=CC=1)(C1C=CC=CC=1)C1C=CC=CC=1)[P](C1C=CC=CC=1)(C1C=CC=CC=1)C1C=CC=CC=1.O>[C:1]([C:5]1[CH:10]=[CH:9][C:8]([C:33]2[C:32]([S:31][C:30]3[N:21]([CH2:20][CH2:19][CH2:18][NH:17][CH:15]([CH3:16])[CH3:14])[C:22]4[C:23]([N:29]=3)=[C:24]([NH2:28])[N:25]=[CH:26][N:27]=4)=[CH:37][C:36]3[O:38][CH2:39][O:40][C:35]=3[CH:34]=2)=[CH:7][CH:6]=1)([CH3:4])([CH3:3])[CH3:2]
KomponentenReaktionsbedingungenVersuchsvorschriftAufarbeitungQuellen und NachweiseNotizen zur Reaktion

Komponenten

7

Edukt 4

HCID 6228DimethylformamideHCID 516892Sodium BicarbonateHCID 27343204-tert-Butylphenylboronic acidHCID 9549213Zelavespib

Produkt 1

HCID 671159988-(6-(4-tert-butylphenyl)benzo[d][1,3]-dioxol-5-ylthio)-9-(3-(isopropylamino)propyl)-9H-purin-6-amineAusbeute 83 %

Reagenzien, Katalysatoren, Lösungsmittel 2

HCID 178418476HCID 178418476, VerbindungKatalysatorHCID 962WaterLösungsmittel

Reaktionsbedingungen

Temperatur
90 °C

Versuchsvorschrift

4-tert-Butylphenylboronic acid (15.6 mg, 0.0876 mmol) was added to PU-H71 (30 mg, 0.0585 mmol) and NaHCO3 (14.7 mg, 0.1755 mmol). DMF (0.5 mL) was added and the reaction mixture was evacuated and back filled with nitrogen. This was repeated four times then nitrogen was bubbled through the reaction mixture for 10 minutes. Then H2O (0.1 mL) and Pd(PPh3)2Cl2 (4 mg, 0.00584 mmol) were added and the reaction mixture was heated under nitrogen at 90° C. for 3 h. Solvent was removed under reduced pressure and the resulting residue was purified by preparatory TLC (hexane:CH2Cl2:EtOAc:MeOH—NH3 (7N), 4:5:2:1) to give 25.0 mg (83%) of DZ2-390. 1H NMR (500 MHz, MeOH-d4) δ 8.11 (s, 1H), 7.27 (d, J=8.4 Hz, 2H), 7.14 (s, 1H), 7.12 (d, J=8.4 Hz, 2H), 6.86 (s, 1H), 6.06 (s, 2H), 3.93 (t, J=6.9 Hz, 2H), 2.92 (septet, J=6.5 Hz, 1H), 2.61 (t, J=7.3 Hz, 2H), 1.86 (m, 2H), 1.28 (s, 9H), 1.12 (d, J=6.5 Hz, 6H); 13C NMR (125 MHz, MeOH-d4) δ 155.9, 153.3, 151.9, 151.8, 150.9, 150.2, 149.2, 141.9, 138.8, 130.0, 125.9, 120.4, 120.3, 115.4, 112.3, 103.6, 50.6, 44.0, 41.8, 35.4, 31.8, 29.3, 21.1; HRMS (ESI) m/z [M+H]+ calcd. for C28H35N6O2S, 519.2542; found. 519.2545; HPLC: method A Rt=7.43, method B Rt=9.45.

Aufarbeitung

  1. customthe reaction mixture was evacuated
  2. additionback filled with nitrogen
  3. customnitrogen was bubbled through the reaction mixture for 10 minutes
  4. customSolvent was removed under reduced pressure
  5. customthe resulting residue was purified by preparatory TLC (hexane:CH2Cl2:EtOAc:MeOH—NH3 (7N), 4:5:2:1)

Quellen und Nachweise

ORD-Eintrag
ord-cf6025c3ba524287934d6f3c7c9946ec
Quelldatensatz
uspto-grants-2016_05
DOI
10.6084/m9.figshare.5104873.v1
Patentnummer
US09328114B2

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