N-Succinimidyl 3-(2-pyridyldithio)propionate → Spdp dtt

HRID 538911

Reaktionsgleichung

Reaktionsgleichung von HRID 538911
[CH2:1]1[C:6](=[O:7])[N:5]([O:8][C:9]([CH2:11][CH2:12][S:13][S:14][C:15]2[N:20]=[CH:19][CH:18]=[CH:17][CH:16]=2)=[O:10])[C:3](=[O:4])[CH2:2]1.[CH2:21]([SH:28])[C@@H:22]([OH:27])[C@H:23]([OH:26])[CH2:24][SH:25].C1C(CN2C(=O)C=CC2=O)CCC(C(ON2C(=O)CCC2=O)=O)C1>>[CH2:1]1[C:6](=[O:7])[N:5]([O:8][C:9]([CH2:11][CH2:12][S:13][S:14][C:15]2[N:20]=[CH:19][CH:18]=[CH:17][CH:16]=2)=[O:10])[C:3](=[O:4])[CH2:2]1.[CH2:21]([SH:28])[C@@H:22]([OH:27])[C@H:23]([OH:26])[CH2:24][SH:25]

Komponenten

4

Edukt 3

HCID 100682N-Succinimidyl 3-(2-pyridyldithio)propionateHCID 125175Succinimidyl 4-(N-maleimidomethyl)cyclohexanecarboxylateHCID 446094D-1,4-Dithiothreitol

Produkt 1

HCID 131730073Spdp dtt

Versuchsvorschrift

SPDP-modified/DTT-reduced streptavidin is mixed with SMCC-modified BLA (equimolar ratio) and allowed to react for 1-2 hrs RT (or overnight at 4 degrees). The streptavidin-BLA conjugate product is purified by gel filtration over P-60 resin.

Aufarbeitung

  1. customto react for 1-2 hrs RT (or overnight at 4 degrees)
  2. filtrationThe streptavidin-BLA conjugate product is purified by gel filtration over P-60 resin

Quellen und Nachweise

ORD-Eintrag
ord-4477b41de58a4a6a9962a17f986ebdad
Quelldatensatz
uspto-grants-2014_10
Patentnummer
US08865891B2

Verknüpfte Notizen

Wird geladen…