N-Hydroxysuccinimido-d-biotin → Biotin

Beteiligte Stoffe

Rolle	Name	HCID	Einwaage
Edukt	N-Hydroxysuccinimido-d-biotin	HCID 129676281	—
Lösungsmittel	Dimethyl Sulfoxide	HCID 679	—
Produkt	Biotin	HCID 171548	—

Reaktionsschritte

Dissolve 1 mg of N-hydroxysuccinimidobiotin (bio-NHS) in 1 ml dimethylsulfoxide. Add 7 ul of bio-NHS solution to protein (a 3 fold molar excess). Let sit 1 hr at room temperature. Dialyze vs PBS.

Herkunft & Nachweise

DOI	10.6084/m9.figshare.5104873.v1
Patentnummer	US04676980
Quelldatensatz	uspto-grants-1987_06
ORD ID	ord-460124e2cad44e45925ff2c99d38b852
Quellennotiz	uspto-grants-1987_06