Phosphoric Acid → (2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)ammonium dihydrogen phosphate

HRID 727766Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 727766
[CH2:1]([OH:8])[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3][OH:4].[P:9](=[O:13])([OH:12])([OH:11])[OH:10]>O>[CH2:1]([OH:8])[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3][OH:4].[OH:11][P:9]([OH:13])([OH:12])=[O:10]

Komponenten

4

Edukt 2

HCID 1004Phosphoric AcidHCID 6503Tris(hydroxymethyl)aminomethane

Produkt 1

HCID 3083992(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)ammonium dihydrogen phosphate

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 962WaterLösungsmittel2×

Versuchsvorschrift

A sample of well agitated fermentation mixture (5.0 ml) is centrifuged for five minutes at 17,000 rpm in a centrifuge tube, the supernate decanted and discarded, and the pellet resuspended in 4.9 ml of 0.1M tris phosphate buffer. The tris phosphate buffer is prepared by dissolving 12.1 grams of Sigma Chem. Co. T-1503 Trizma base in 800 ml of deionized water, adjusting the pH at 25° C. to 7.5 with phosphoric acid and diluting with deionized water to a final volume of 1000 ml.

Aufarbeitung

  1. customthe supernate decanted
  2. dissolutionby dissolving 12.1 grams of Sigma Chem
  3. customat 25° C.

Quellen und Nachweise

ORD-Eintrag
ord-5b9c7780e36b4c9391c0eaef9d762d50
Quelldatensatz
uspto-grants-1991_09
Patentnummer
US05045465

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