Bicarbonate Ion → Biotin

HRID 7341

Reaktionsgleichung

Reaktionsgleichung von HRID 7341
[C:1](=[O:4])(O)[O-:2].[CH2:5]1[S:9][C@@H:8]([CH2:10][CH2:11][CH2:12][CH2:13]C(NCCCCCC(ON2C(=O)C(S([O-])(=O)=O)CC2=O)=O)=O)[C@H:7]2[NH:36][C:37]([NH:39][C@@H:6]12)=[O:38].[Na+]>O>[OH:2][C:1]([CH2:13][CH2:12][CH2:11][CH2:10][C@H:8]1[C@@H:7]2[C@@H:6]([NH:39][C:37]([NH:36]2)=[O:38])[CH2:5][S:9]1)=[O:4]

Komponenten

4

Edukt 2

HCID 769Bicarbonate IonHCID 71571497Sulphosuccinimidyl-6-(biotinamido)hexanoate

Produkt 1

HCID 171548Biotin

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 962WaterLösungsmittel

Versuchsvorschrift

About 2.0 mg of DBP is buffer exchanged with 20 mM bicarbonate at pH 9.6 three times to give a final volume of 1.0 mL. Two aliquots of sulfo-NHS-LC-biotin (Pierce) are dissolved in water to give a 1 mg/mL solution just prior to addition. The first aliquot (10 uL) is added and the reaction is maintained at room temperature for 5 minutes. The second aliquot (10 uL) is added and the reaction proceeds for an additional 4 minutes. The unreacted biotin is removed by size exclusion chromatography using SEPHADEX G-25 and 100 mM PBS, pH 7.4 as the mobile phase.

Aufarbeitung

  1. customto give a final volume of 1.0 mL
  2. customto give a 1 mg/mL solution just
  3. additionto addition
  4. additionThe first aliquot (10 uL) is added
  5. additionThe second aliquot (10 uL) is added
  6. customThe unreacted biotin is removed by size exclusion chromatography

Quellen und Nachweise

ORD-Eintrag
ord-34edcaa7e3ba4173928e329654c5e7d9
Quelldatensatz
uspto-grants-2006_08
Patentnummer
US07087395B1

Verknüpfte Notizen

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