Phosphate → Glyoxal 2'-deoxyguanosine

HRID 857828

Reaktionsgleichung

Reaktionsgleichung von HRID 857828
[C@@H:1]1(N2C=CC(=O)NC2=O)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1.C(C=O)=O.[NH:21]1[C:30](=[O:31])[C:29]2[NH:28][CH:27]=[N:26][C:25]=2[N:24]=[C:22]1[NH2:23].P([O-])([O-])([O-])=O.B(O)(O)O.C(N(CC(O)=O)CC(O)=O)CN(CC([O-])=O)CC([O-])=O.[Na+].[Na+]>>[CH:3]([CH:5]=[O:8])=[O:4].[C@@H:1]1([N:26]2[C:25]3[N:24]=[C:22]([NH2:23])[NH:21][C:30](=[O:31])[C:29]=3[N:28]=[CH:27]2)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1

Komponenten

6

Edukt 5

HCID 1061PhosphateHCID 7628Boric AcidHCID 13712Deoxyuridine2×HCID 13020083Ethylenediaminetetraacetic acid disodium saltHCID 136394281Glyoxal guanine2×

Produkt 1

HCID 136394279Glyoxal 2'-deoxyguanosine

Versuchsvorschrift

Glyoxal-2′-deoxyguanosine was synthesized from 2′-deoxyuridine and glyoxal-guanine under the following conditions, by using any one of the aforementioned three sets of enzyme solutions and the Bacillus stearothermophilus JTS 859 enzyme solution obtained in Production Example 2. Specifically, 5 mM phosphate buffer (pH 7) containing 30 mM 2′-deoxyuridine, 30 mM glyoxal-guanine, 100 mM boric acid, and 4 mM disodium ethylenediaminetetraacetate was first prepared.

Aufarbeitung

  1. customthe Bacillus stearothermophilus JTS 859 enzyme solution obtained in Production Example 2
  2. customwas first prepared

Quellen und Nachweise

ORD-Eintrag
ord-dc43600221a54b83ac5cb8d4a912c25b
Quelldatensatz
uspto-grants-2006_12
Patentnummer
US07148042B2

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