NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

HRID 864980

Reaktionsgleichung

Reaktionsgleichung von HRID 864980
C(O)[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.N[C@@H](CCC(N[C@H](C(NCC(O)=O)=O)CS)=O)C(O)=O.C(N(CC([O-])=O)CC(O)=O)CN(CC([O-])=O)CC(O)=O.[Na+].[Na+].[CH:66]1[CH:71]=[N+:70]([C@@H:72]2[O:76][C@H:75]([CH2:77][O:78][P:79]([O:82][P:83]([O:86][CH2:87][C@H:88]3[O:92][C@@H:91]([N:93]4[C:97]5[N:98]=[CH:99][N:100]=[C:101]([NH2:102])[C:96]=5[N:95]=[CH:94]4)[C@H:90]([O:103][P:104]([OH:107])([OH:106])=[O:105])[C@@H:89]3[OH:108])([OH:85])=[O:84])([OH:81])=[O:80])[C@@H:74]([OH:109])[C@H:73]2[OH:110])[CH:69]=[C:68]([C:111]([NH2:113])=[O:112])[CH:67]=1.[Cl-].[Mg+2].[Cl-].C(OP(O)(O)=O)[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O.P(OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)(O)(O)=O>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[CH:99]1[N:100]=[C:101]([NH2:102])[C:96]2[N:95]=[CH:94][N:93]([C@@H:91]3[O:92][C@H:88]([CH2:87][O:86][P:83]([O:82][P:79]([O:78][CH2:77][C@H:75]4[O:76][C@@H:72]([N:70]5[CH:69]=[C:68]([C:111]([NH2:113])=[O:112])[CH2:67][CH:66]=[CH:71]5)[C@H:73]([OH:110])[C@@H:74]4[OH:109])([OH:81])=[O:80])([OH:85])=[O:84])[C@@H:89]([OH:108])[C@H:90]3[O:103][P:104]([OH:107])([OH:106])=[O:105])[C:97]=2[N:98]=1

Komponenten

9

Edukt 7

HCID 5886NADP nicotinamide-adenine-dinucleotide phosphateHCID 7427TrehaloseHCID 8759EDTA disodium saltHCID 82400Glucose 1,6-bisphosphateHCID 124886GlutathioneHCID 439958Glucose-6-PhosphateHCID 5360315Magnesium Chloride

Produkt 1

HCID 5884NADPH

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 62657Potassium Phosphate, TribasicLösungsmittel

Versuchsvorschrift

The enzymic activity is determined according to the following procedure: 10.8 g of trehalose, 860 mg of glutathione, and 17.2 mg of Na2EDTA are dissolved in a 57 mM potassium phosphate buffer solution (pH 7.0) to prepare 100 ml of a solution. A mixture of 1.40 ml of the thus prepared solution, 100 μl of a 20 mM NADP+ solution, 100 μl of a 26 mM magnesium chloride solution, 100 μl of a 1.34 mM glucose 1,6-diphosphate solution, 100 μl of a 31 unit/ml phosphoglucomutase solution, 100 μl of a 35 unit/ml glucose 6-phosphate dehydrogenase solution, and 100 μl of a sample solution is incubated at 30° C. and the quantity of produced NADPH is monitored consecutively by means of absorption spectrophotometry at 340 nm. One unit refers to the amount of enzyme which is capable of producing 1 μmol of NADPH for 1 min. under the conditions.

Aufarbeitung

  1. customis incubated at 30° C.
  2. customthe quantity of produced NADPH is monitored consecutively by means of absorption spectrophotometry at 340 nm

Quellen und Nachweise

ORD-Eintrag
ord-0aa56980d7364ae7ad0b1e2dbdea3779
Quelldatensatz
uspto-grants-1996_10
Patentnummer
US05565341

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