NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

HRID 864982

Reaktionsgleichung

Reaktionsgleichung von HRID 864982
P(O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)(O)(O)=O.C(N(CC([O-])=O)CC(O)=O)CN(CC([O-])=O)CC(O)=O.[Na+].[Na+].[CH:39]1[CH:44]=[N+:43]([C@@H:45]2[O:49][C@H:48]([CH2:50][O:51][P:52]([O:55][P:56]([O:59][CH2:60][C@H:61]3[O:65][C@@H:64]([N:66]4[C:70]5[N:71]=[CH:72][N:73]=[C:74]([NH2:75])[C:69]=5[N:68]=[CH:67]4)[C@H:63]([O:76][P:77]([OH:80])([OH:79])=[O:78])[C@@H:62]3[OH:81])([OH:58])=[O:57])([OH:54])=[O:53])[C@@H:47]([OH:82])[C@H:46]2[OH:83])[CH:42]=[C:41]([C:84]([NH2:86])=[O:85])[CH:40]=1.[Cl-].[Mg+2].[Cl-].C(OP(O)(O)=O)[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O.P(OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)(O)(O)=O>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[CH:72]1[N:73]=[C:74]([NH2:75])[C:69]2[N:68]=[CH:67][N:66]([C@@H:64]3[O:65][C@H:61]([CH2:60][O:59][P:56]([O:55][P:52]([O:51][CH2:50][C@H:48]4[O:49][C@@H:45]([N:43]5[CH:42]=[C:41]([C:84]([NH2:86])=[O:85])[CH2:40][CH:39]=[CH:44]5)[C@H:46]([OH:83])[C@@H:47]4[OH:82])([OH:54])=[O:53])([OH:58])=[O:57])[C@@H:62]([OH:81])[C@H:63]3[O:76][P:77]([OH:80])([OH:79])=[O:78])[C:70]=2[N:71]=1

Komponenten

8

Edukt 6

HCID 5886NADP nicotinamide-adenine-dinucleotide phosphateHCID 8759EDTA disodium saltHCID 65533alpha-D-glucose 1-phosphateHCID 82400Glucose 1,6-bisphosphateHCID 439958Glucose-6-PhosphateHCID 5360315Magnesium Chloride

Produkt 1

HCID 5884NADPH

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 62657Potassium Phosphate, TribasicLösungsmittel

Versuchsvorschrift

The concentration of α-glucose 1-phosphate is determined according to the following procedure: 17.1 mg of Na2EDTA is dissolved in 47 mM potassium phosphate buffer (pH 7.0) to prepare 100 ml of a solution. A mixture of 2.5 ml of the solution, 100 μl of a 14.8 mM NADP+ solution, 100 μl of a 26 mM magnesium chloride solution, 100 μl of a 1.34 mM glucose 1,6-diphosphate solution, 50 μl of a 31 unit/ml phosphoglucomutase solution, 50 μl of a 35 unit/ml glucose 6-phosphate dehydrogenase solution, and 100 μl of a sample solution is incubated at 30° C. for 30 min. and subjected to spectrophotometric measurement at 340 nm to give the amount of produced NADPH, thus determining indirectly the amount of α-glucose 1-phosphate in the sample solution.

Quellen und Nachweise

ORD-Eintrag
ord-92eafb894147484aae9142c746488987
Quelldatensatz
uspto-grants-1996_10
Patentnummer
US05565341

Verknüpfte Notizen

Wird geladen…