Basic information
- CAS
2044-85-1- Standard SMILES
CC(=O)Oc1cc2c(cc1Cl)C1(OC(=O)c3ccccc31)c1cc(Cl)c(OC(C)=O)cc1O2- InChIKey
VQVUBYASAICPFU-UHFFFAOYSA-N- Molecular formula
- C24H14Cl2O7
- Average molar mass
- 485.3 g/mol
- Monoisotopic mass
- 484.0116582
Names & identifiers
- Preferred name
- Diacetyldichlorofluorescein
- IUPAC
- (6'-acetyloxy-2',7'-dichloro-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate
- English name
- 2',7'-Dichlorofluorescin diacetate
- Molecular formula
- C24H14Cl2O7
- Molecular weight
- 485.27
Names & synonyms
2044-85-12',7'-Dichlorofluorescein diacetateDiacetyldichlorofluoresceinDCFDALeuco-dcf diacetateLeukodiacetyl-2,7-dichlorofluoresceinDTXSID30174389RefChem:132565DTXCID70968802',7'-Dichlorofluorescin diacetateDcfh-DA2',7'-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate
Show more names
H2DCFDA2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATEDCFH2-DAMFCD 37501DCDHF DIACETATESynonyms: DCFH-DADiacetyldichlorofluoresceDIACETYLDICHLOROFLUORESCIN
(6'-acetyloxy-2',7'-dichloro-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetateMFCD000375012 inverted exclamation marka,7 inverted exclamation marka-Dichlorofluorescein diacetateSpiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-2',7'-dichloro-(6'-acetoxy-2',7'-dichloro-3-oxo-spiro[isobenzofuran-1,9'-xanthene]-3'-yl) acetate2',7'-dichloro-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetateC24H14Cl2O7Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-bis(acetyloxy)-2',7'-dichloro-
Properties
Computed structural properties
- XLogP
- 4.9
- Topological polar surface area
- 88.1 Ų
- Hydrogen bond donors
- 0
- Hydrogen bond acceptors
- 7
- Rotatable bonds
- 4
- Heavy atoms
- 33
- Formal charge
- 0
- Complexity
- 775
Physicochemical properties
- Melting point
- 232 · 232-234 °C(lit.)
- Boiling point
- 657.9 · 657.9±55.0 °C(Predicted)
- Density
- 1.59 · 1.59±0.1 g/cm3(Predicted)
- Flash point
- 109 ℃ · >109℃
- storage temp.
- 2 · 2-8°C
- solubility
- DMSO: soluble
- form
- Powder or Crystals
- color
- Colorless to off-white
- Appearance
- Solid Powder
- BRN
- 68488 · 68488
- InChI
- InChI=1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
- InChIKey
- VQVUBYASAICPFU-UHFFFAOYSA-N
- SMILES
- C12(C3=C(C=C(OC(C)=O)C(Cl)=C3)OC3=C1C=C(Cl)C(OC(C)=O)=C3)C1=C(C=CC=C1)C(=O)O2
- UNSPSC Code
- 12352108 · 12352108
- NACRES
- 32 · NA.32
- Symbol(GHS)
Safety & regulation
Safety data
- F
- 3-8-10
- PPE
- dust mask type N95 (US), Eyeshields, Gloves
- wgk
- 3
- ridadr
- 1759
- hs_code
- 29329990
- risk_code
- 36/37/38-34-20/22
- Signal word
- Warning
- Symbol(GHS)
- GHS07
- hazard_code
- Xi
- safety_code
- 26-36-45-36/37/39
- Storage Class
- 11 - Combustible Solids
- Hazard statements
- H315-H319-H335
- Precautionary statements
- P261-P305+P351+P338
Chemistry & industry
Applications
Description2',7'-Dichlorofluorescein diacetate is a cell-permeable fluorogenic probe for detecting reactive oxygen species (ROS) and nitric oxide (NO).
UsesCell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalyzed cleavage of the diacetate groups, DCDHF is oxidized by peroxynitrite yielding the highly fluorescent product
Uses2′,7′-Dichlorofluorescein diacetate may be used as a superior gas phase alternative to underivatized fluorescein and as a component/substrate of the 2′,7′-dichlorofluorescein diacetate assay to quantitate reactive oxygen species (ROS).
Upstream & downstream products
Downstream products
Suppliers94 total
国药集团化学试剂有限公司86-21-63210123 · [email protected]
上海阿拉丁生化科技股份有限公司400-6206333 13167063860 · [email protected]
湖南韵邦生物科技股份有限公司85561037 15386497078 · [email protected]
Career Henan Chemica Co+86-0371-86658258 +86-13203830695 · [email protected]
上海哲研生物科技有限公司021-18017610038 18017610038 · [email protected]
上海优宁维生物科技股份有限公司
Related reactions
1 records
Compound notes
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