Potassium Chloride → Quercetin

HRID 2420677

Reaction equation

HRID 2420677 reaction equation
C([O-])(=O)CC(CC([O-])=O)(C([O-])=O)[OH:4].O=[CH:15][C@@H:16]([C@H:18]([C@@H:20]([C@@H:22]([CH2:24][OH:25])O)[OH:21])O)[OH:17].CC1C=CC(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(OCC[O:55][C:56]2[CH:61]=[C:60]3[CH:62]=[C:63]([C:65]4[O:69]C(C(OCOC(C)=O)=O)=CN=4)[O:64][C:59]3=[CH:58][C:57]=2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=1.CC(OC1C=C2OC3C(C4(OC(=O)C5C4=CC=CC=5)C2=CC=1Cl)=CC(Cl)=C(OC(C)=O)C=3)=O.[Na+].[Cl-].[Cl-].[K+].[Mg+2].[Cl-].[Cl-].C1N(CCO)CCN(CCS(O)(=O)=O)C1>CO>[CH:59]1[C:60]([C:62]2[O:17][C:16]3[CH:15]=[C:24]([OH:25])[CH:22]=[C:20]([OH:21])[C:18]=3[C:65](=[O:69])[C:63]=2[OH:64])=[CH:61][C:56]([OH:55])=[C:57]([OH:4])[CH:58]=1

Participants

10

Reactant 8

HCID 4873Potassium ChlorideHCID 5234Sodium ChlorideHCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acidHCID 31348CitrateHCID 104913DiacetyldichlorofluoresceinHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×HCID 3364574Fura 2amHCID 5360315Magnesium Chloride

Product 1

HCID 5280343Quercetin

Reagents, catalysts & solvents 1

HCID 887MethanolSolvent

Procedure

Drug-free human blood obtained from healthy volunteers was coagulated with acid:citrate:dextrose. Platelet-rich plasma was centrifuged at 800×g for 20 min at room temperature and the pellet was suspended in a volume equal to half the initial volume of autologous plasma, low in platelets. The platelet suspensions were incubated for 1 h at 37° C. with 3 μmol of Fura 2/AM per L, 40 μmol DCFH-DA/L, 7.4 GBq (2 Ci) 32Pi/L, or 3.7 MBq (1 mCi) [3H]oleic acid/L. The platelets were washed by exclusion chromatography on Sepharose 2B using a Ca2+-free Tyrodes buffer (134 mmol NaCl/L, 2.9 mmol KCl/L, 0.34 mmol Na2HPO4/L and 2 mmol MgCl2/L) containing 0.2% of bovine serum albumin, 5 mmol glucose/L and 10 mmol HEPES/L, pH 7.35. The platelets that had been submitted to exclusion chromatography (PSEC) were adjusted to a final concentration of 2×1011 cells/L. Since the addition of methanol to the suspensions of PSEC at concentrations<0.5% did not cause any change in the response of the PSEC to collagen, this ratio was used for obtaining final concentrations of quercetin that varied between 5 and 20 μmol/L. Catechin and quercetin were added to the suspensions of PSEC while stirring continuously for 30 min at 37° C. and then removed by centrifugation at 800×g for 20 min at room temperature.

Workup

  1. customDrug-free human blood obtained from healthy volunteers
  2. washThe platelets were washed by exclusion chromatography on Sepharose 2B
  3. concentrationat concentrations<0.5%

Sources & evidence

ORD record
ord-1555a0ea6f3445fc9748f950494d5e7b
Source dataset
uspto-grants-2011_06
Patent number
US07956040B2

Linked notes

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