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Basic information
- CAS
9014-74-8- Standard SMILES
CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccc(F)cc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccc(F)cc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccccc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccccc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)c1ccccc1.CC(C)CC(C=Cc1ccc(C(C)(C)C)cc1)CCCCS(=O)(=O)O.COc1ccc(CC(C=CC(C)(C)C)CCCCS(=O)(=O)O)cc1.COc1ccc(CC(C=CC(C)(C)C)CCCCS(=O)(=O)O)cc1- Molecular formula
- NULL
Names & identifiers
- Preferred name
- ENTEROKINASE
- English name
- ENTEROKINASE
- Molecular formula
- NULL
- Molecular weight
- 0
Names & synonyms
RECOMBINANT ENTEROKINASEEC 3.4.21.9ENTEROPEPTIDASEREKENTKPRSS7TMPRSS15ENTEROKISEENTEROKINASEEnterrokinase
Database identifiers
- CAS
9014-74-8- PubChem CID
- 168009870
- EC Number
- 232-761-1
Properties
Physicochemical properties
- storage temp.
- -20 °C · -20°C
- form
- salt-free, lyophilized powder
- color
- white
- biological source
- bovine intestine
- Specific Activity
- 20 units/mg · ≥20units/mg protein
- CAS DataBase Reference
- 9014 · 9014-74-8
- FDA UNII
- ENTEROPEPTIDASE (PORCINE) (1N6UU71ZTI)ENTEROPEPTIDASE (HUMAN) (228MAT8QUT)ENTEROPEPTIDASE (BOVINE) (7QBB4Y898A)
- EPA Substance Registry System
- Peptidase, entero- (9014-74-8)
- UNSPSC Code
- 41116107 · 41116107
- NACRES
- 56 · NA.56
- Symbol(GHS)
- 7 · GHS07,GHS08
- Signal word
- Danger
- Hazard statements
- 315 · H315-H319-H334-H335
- Precautionary statements
- 261 · P261-P284-P304+P340-P305+P351+P338-P342+P311
- PPE
- 95 · Eyeshields, Gloves, type N95 (US)
- WGK Germany
- 3 · 3
- TSCA
- TSCA listed
- Storage Class
- 11 · 11 - Combustible Solids
Safety & regulation
Safety information is for reference only and does not replace the supplier’s Safety Data Sheet (SDS), applicable regulations, or professional assessment.
Safety data
- PPE
- Eyeshields, Gloves, type N95 (US)
- wgk
- 3
- tsca
- TSCA listed
- Signal word
- Danger
- Symbol(GHS)
- GHS07,GHS08
- Storage Class
- 11 - Combustible Solids
- Hazard statements
- H315-H319-H334-H335
- Precautionary statements
- P261-P284-P304+P340-P305+P351+P338-P342+P311
Chemistry & industry
Applications
UsesTypical conditions for fusion protein cleavage:Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl2, and 1% Tween 20 Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mixIncubate reaction mixture at ~25 °C for 16 hours
General DescriptionEnterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.
DescriptionEnterokinase (rEK) Bovine Recombinant is the catalytic subunit of bovine enterokinase, which is expressed by E. Coli and purified to yield a high enzyme activity preparation. EK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence. Recombinant Bovine Enterokinase is a single glycosylated polypeptide chain containing 235 amino acids and having an MW of ~28kDa.
Suppliers98 total
上海阿拉丁生化科技股份有限公司400-6206333 13167063860 · [email protected]
湖南韵邦生物科技股份有限公司0731-88532326 13319508186 · [email protected]
南通飞宇生物科技有限公司0513-68819626 15152877458 · [email protected]
上海康朗生物科技有限公司021-61998208 18217752821 · [email protected]
上海钰博生物科技有限公司021-60514606 18321282235 · [email protected]
上海百泰欣生物有限公司13701899122 · [email protected]
上海哲研生物科技有限公司021-18017610038 18017610038 · [email protected]
Related reactions
0 records
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Compound notes
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