YoYo-1
YOYO 1 · YODi-1 · DiYO?-1 · Chebi:51224 · Yoyo 1 Iodide · YOYO?*-1 equivalent · YOYO-1(1mM in DMSO) · YOYO 1 Green Fluorescent Dye
Identifiers & Physical Properties
| CAS No. | 143413-85-8 |
|---|---|
| Molecular formula | C49H58I4N6O2 |
| Average molar mass | 1,270.6 g/mol |
| Monoisotopic mass | 1,270.08 g/mol |
| SMILES | CN1/C(=C/c2cc[n+](CCC[N+](C)(C)CCC[N+](C)(C)CCC[n+]3ccc(/C=C4/Oc5ccccc5N4C)c4ccccc43)c3ccccc23)Oc2ccccc21.[I-].[I-].[I-].[I-] |
| InChIKey | GRRMZXFOOGQMFA-UHFFFAOYSA-J |
| PubChem CID | 6438136 |
| DTXSID | DTXSID801030161 |
Chemical & Physical Properties
| Topological polar surface area | 32.7 |
|---|---|
| Hydrogen bond donors | 0 |
| Hydrogen bond acceptors | 8 |
| Rotatable bonds | 14 |
| Heavy atoms | 61 |
| Formal charge | 0 |
| Complexity | 1,260 |
| Chemical classes | Other Uses -> Biochemical Research |
| Physical Description | Yellow liquid[Molecular Probes MSDS] |
| Melting point | >250 ℃ |
| Solubility | Soluble in dimethyl sulfoxide, water |
| Physical state | powder |
| Color | Orange-red |
| Appearance | orange solution |
| ex/em | 491/509 nm |
| ε(extinction coefficient) | 98900 cm−1M−1 |
| Φ(quantum yield) | 0.52 |
| λmax | 491 nm |
| Biological Applications | Nucleic acid hybridizationnucleic acid sequencingdetecting nucleic acids,cells,human papilloma virus (HPV),pathogens,spores,cancer cells,stress biomarkers,viruses |
| FDA UNII | 1B34829IG0 |
Safety & Regulations
| Hazards summary | Possible mutagen[Molecular Probes MSDS] |
|---|
Applications & Uses
Description
YODi-1 (Oxazole Yellow Homodimer, also known as YOYO®-1) is a green fluorescent carbocyanine dimeric dye. YODi-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits over a thousand-fold fluorescence enhancement upon binding to dsDNA.The bright fluorescence signal and low background make YODi-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments. YODi-1 can also be used for the analysis of single molecules of DNA.
Uses
Thiazole orange dimer YOYO 1 is a green fluorescent dye used for DNA staining. It belongs to the monomethine cyanine dye family and is a tetracationic homodimer of oxazole yellow (abbreviated as YO, hence the name YOYO), usually provided as a tetraiodide salt. In aqueous buffer, the free YOYO-1 dye (λmax 458 nm; λmax 564 nm) has a very low fluorescence quantum yield, but after binding to double-stranded DNA through diintercalation, the fluorescence intensity is increased by 3200 times (λmax 489 nm; λmax 509 nm).