Tricine
Basic information
- CAS
5704-04-1- Standard SMILES
O=C(O)CNC(CO)(CO)CO- InChIKey
SEQKRHFRPICQDD-UHFFFAOYSA-N- Molecular formula
- C6H13NO5
- Average molar mass
- 179.17 g/mol
- Monoisotopic mass
- 179.07937252
Overview
N-tris(hydroxymethyl)methylglycine is a Good's buffer substance, pKa = 8.15 at 20 C. It is functionally related to a glycine and a member of tris. It is a tautomer of a N-tris(hydroxymethyl)methylammonioacetate.
Names & identifiers
- Preferred name
- Tricine
- IUPAC
- 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
- English name
- Tricine
- Molecular formula
- C6H13NO5
- Molecular weight
- 179.17
Names & synonyms
Tricine5704-04-1N-Tris(hydroxymethyl)methylglycineN-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycineGlycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-2-((1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl)amino)acetic acidW12LH4V8V3NSC-3699952-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acidDTXSID1063982CHEBI:39063n-(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)glycine
Show more names
TRIS-HCL BUFFER1389475TROMETHAMINE HYDROCHLORIDETicineTricine buffer, free acidTRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDETRIClNEn-Tricine-D8
2-{[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}acetic acidGlycine, N-(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)-RefChem:899271DTXCID6042159CHEBI:467602-((1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl)azaniumyl)acetate227-193-6N-[Tris(hydroxymethyl)methyl]glycine
Database identifiers
- CAS
5704-04-1- PubChem CID
- 79784
- DTXSID
DTXSID1063982- ChEBI
CHEBI:39063- UNII
- W12LH4V8V3
- EC Number
- 227-193-6
- Nikkaji
- J135.902I
Properties
Computed structural properties
- XLogP
- -5.1
- Topological polar surface area
- 110 Ų
- Hydrogen bond donors
- 5
- Hydrogen bond acceptors
- 6
- Rotatable bonds
- 6
- Heavy atoms
- 12
- Formal charge
- 0
- Complexity
- 137
Experimental & physicochemical properties
Chemical ClassesOther Uses -> Biological Buffers
Physical DescriptionSolid; [Merck Index] White crystals; [Sigma-Aldrich MSDS]
Physicochemical properties
- Melting point
- 186 · 186-188 °C (dec.)
- Boiling point
- 311.69 °C · 311.69°C (rough estimate)
- Density
- 1.05 g/mL · 1.05 g/mL at 20 °C
- bulk density
- 500 kg/m3 · 500kg/m3
- vapor pressure
- 0.002 · 0.002-0.004Pa at 20-25℃
- refractive index
- 1.424 · 1.4240 (estimate)
- storage temp.
- Keep in dark place,Inert atmosphere,Room temperature
- solubility
- 2 O: · H2O: 4 M at 20 °C, clear, colorless
- form
- crystalline
- color
- clear colorless (40 % (w/w) in H2O) solution
Safety & regulation
Safety information is for reference only and does not replace the supplier’s Safety Data Sheet (SDS), applicable regulations, or professional assessment.
GHS classification
GHS Classification
This chemical does not meet GHS hazard criteria for 97.6% (121 of 124) of all reports.
Not Classified
Aggregated GHS information provided per 124 reports by companies from 4 notifications to the ECHA C&L Inventory.
Hazard information
Regulatory Information
Chemical: Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-
REACH registered substance: N-(tri(hydroxymethyl)methyl)glycine (EC: 227-193-6)
Glycine, N-2-hydroxy-1,1-bis(hydroxymethyl)ethyl-: Does not have an individual approval but may be used under an appropriate group standard
Other Safety InformationEvaluation - Chemicals that are unlikely to require further regulation to manage risks to environment
Hazards SummaryMay cause irritation; [Sigma-Aldrich MSDS]
Hazard Classes and CategoriesNot Classified
Safety data
- F
- 3
- wgk
- 3
- tsca
- TSCA listed
- hs_code
- 29052900
- risk_code
- 36/37/38
- Signal word
- Warning
- Symbol(GHS)
- GHS07
- hazard_code
- Xi
- safety_code
- 26-36-36/37/39
Chemistry & industry
Uses & manufacturing
UsesUsed as a biological buffer; [Merck Index]
General Manufacturing InformationGlycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-: ACTIVE
Applications
UsesGood's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).
General DescriptionTricine and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.
Upstream & downstream products
Upstream raw materials
Additional notes
DefinitionChEBI: N-tris(hydroxymethyl)methylglycine is a Good's buffer substance, pKa = 8.15 at 20 ℃. It is functionally related to a member of tris and a glycine. It is a tautomer of a N-tris(hydroxymethyl)methylammonioacetate.
Related reactions
9 records
Compound notes
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