Phenol Red → Tricine SDS

HRID 547239

Reaction equation

HRID 547239 reaction equation
[NH:1]([C:6]([CH2:11][OH:12])([CH2:9][OH:10])[CH2:7][OH:8])[CH2:2][C:3]([OH:5])=[O:4].C(O)C(N)(CO)CO.Cl.[CH3:22][CH2:23][CH2:24][CH2:25][CH2:26][CH2:27][CH2:28][CH2:29][CH2:30][CH2:31][CH2:32][CH2:33][O:34][S:35]([O-:38])(=[O:37])=[O:36].[Na+:39].CCN(C1C=CC(/C(/C2C=CC(NC3C=CC(OCC)=CC=3)=CC=2)=C2/C=CC(C=C/2C)=[N+](CC2C=CC=C(S(O)(=O)=O)C=2)CC)=C(C)C=1)CC1C=CC=C(S(O)(=O)=O)C=1.C1C=CC2S(=O)(=O)OC(C3C=CC(O)=CC=3)(C3C=CC(O)=CC=3)C=2C=1>OCC(CO)O>[NH:1]([C:6]([CH2:9][OH:10])([CH2:11][OH:12])[CH2:7][OH:8])[CH2:2][C:3]([OH:5])=[O:4].[CH3:22][CH2:23][CH2:24][CH2:25][CH2:26][CH2:27][CH2:28][CH2:29][CH2:30][CH2:31][CH2:32][CH2:33][O:34][S:35]([O-:38])(=[O:37])=[O:36].[Na+:39]

Participants

7

Reactant 5

HCID 4766Phenol RedHCID 79784TricineHCID 93573Tromethamine hydrochlorideHCID 3423265Sodium Lauryl SulfateHCID 6333920Acid Blue 90

Product 1

HCID 23667616Tricine SDS

Reagents, catalysts & solvents 1

HCID 753GlycerolSolvent

Procedure

20 μl 2× Tricine Sample Buffer—Non-reducing (TSB-NR) (300 mM Tris-HCl, pH 8.45, 24% Glycerol, 8% SDS, 0.005% Coomassie Blue G, 0.005% Phenol Red) were added to 20 μl Pre-stained markers (Bio-Rad), and 20 μl per well were used.

Sources & evidence

ORD record
ord-8563d15ca27b4ed2817cb767ae73f7fc
Source dataset
uspto-grants-2011_05
Patent number
US07939075B2

Linked notes

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