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Sodium Chloride → Leukotriene B4

HRID 10161
Notes
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Reaction equation

HRID 10161 reaction equation
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5]/[CH:6]=[CH:7]\[CH2:8]/[CH:9]=[CH:10]\[CH:11]=[CH:12]\[CH:13]=[CH:14]\[C@@H:15]1[O:17][C@H:16]1[CH2:18][CH2:19][CH2:20][C:21]([OH:23])=[O:22].CCCCC/C=C\C/C=C\C=C\C=C\[C@@H]1[O:40][C@H]1CCCC(OC)=O.[Na+].[Cl-].C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5]/[CH:6]=[CH:7]\[CH2:8][C@@H:9]([OH:40])/[CH:10]=[CH:11]/[CH:12]=[CH:13]/[CH:14]=[CH:15]\[C@@H:16]([OH:17])[CH2:18][CH2:19][CH2:20][C:21]([OH:23])=[O:22]
ParticipantsProcedureSources & evidenceReaction notes

Participants

6

Reactant 4

HCID 5234Sodium ChlorideHCID 6049Ethylenediaminetetraacetic AcidHCID 5280383Leukotriene A42×HCID 6478221methyl 4-[(2S,3S)-3-[(1E,3E,5Z,8Z)-tetradeca-1,3,5,8-tetraenyl]oxiran-2-yl]butanoate

Product 1

HCID 5280492Leukotriene B4

Reagents, catalysts & solvents 1

HCID 62657
Potassium Phosphate, Tribasic
Solvent

Procedure

The LTA4 substrate was freshly prepared by alkaline hydrolysis of LTA4 methyl ester (Cayman Chemical Co., USA). After incubating for 10 minutes in the presence of LTA4 (1 μM in 50 mM Tris-HCl, 0.15 M NaCl, 0.5% BSA, pH 7.4), the reaction is stopped by diluting 1/20 in 0.1 M potassium phosphate buffer containing 1.5 mM NaN2, 0.4 M NaCl, 1 mM EDTA, 0.1% BSA, pH 7.4, −4° C.

Sources & evidence

ORD record
ord-2c4f74d36a3e4147b27538559e142388
Source dataset
uspto-grants-2006_08
DOI
10.6084/m9.figshare.5104873.v1
Patent number
US07094773B2

Reaction notes

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On this pageParticipantsProcedureSources & evidenceReaction notes