Dihydrokaempferol → Kaempferol

HRID 1157103

Reaction equation

C1C(/C=C/C(O)=O)=CC=C(O)C=1.C1C([C@H]2OC3C=C(O)C=C(O)C=3C(=O)C2)=CC=C(O)C=1.[CH:33]1[C:38]([C@H:39]2[O:49][C:48]3[CH:47]=[C:46]([OH:50])[CH:45]=[C:44]([OH:51])[C:43]=3[C:41](=[O:42])[C@@H:40]2[OH:52])=[CH:37][CH:36]=[C:35]([OH:53])[CH:34]=1>>[CH:33]1[C:38]([C:39]2[O:49][C:48]3[CH:47]=[C:46]([OH:50])[CH:45]=[C:44]([OH:51])[C:43]=3[C:41](=[O:42])[C:40]=2[OH:52])=[CH:37][CH:36]=[C:35]([OH:53])[CH:34]=1

Participants

Reactant 3

HCID 122850Dihydrokaempferol

HCID 439246Naringenin

HCID 637542P-Coumaric Acid

Product 1

HCID 5280863Kaempferol

Procedure

We also performed the shake flask experiments in LB medium, under the same conditions as described for the M9 medium. When p-coumaric acid was used as a precursor, naringenin (0.39 mg/lt) and dihydrokaempferol (1.29 mg/lt) accumulated in the fermentation broth but no kaempferol was produced. When caffeic acid was used as a precursor, eriodictyol accumulated at a lower concentration compared to the M9 medium (7.5 μg/lt), while no dihydroquercetin or quercetin was detected. Finally, when cinnamic acid was used, pinocembrin accumulated at a concentration of 0.245 mg/lt and no dihydroflavonol or flavonol was detected.

Sources & evidence

ORD record: ord-20d2b72683364280ab64f4424200053e
Source dataset: uspto-grants-2010_10
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07807422B2

Linked notes

Loading…