Reaction equation
[C@@H:1]1(N2C=CC(=O)NC2=O)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1.C(C=O)=O.[NH:21]1[C:30](=[O:31])[C:29]2[NH:28][CH:27]=[N:26][C:25]=2[N:24]=[C:22]1[NH2:23].P([O-])([O-])([O-])=O.B(O)(O)O.C(N(CC(O)=O)CC(O)=O)CN(CC([O-])=O)CC([O-])=O.[Na+].[Na+]>>[CH:3]([CH:5]=[O:8])=[O:4].[C@@H:1]1([N:26]2[C:25]3[N:24]=[C:22]([NH2:23])[NH:21][C:30](=[O:31])[C:29]=3[N:28]=[CH:27]2)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1Participants
Procedure
Glyoxal-2′-deoxyguanosine was synthesized from 2′-deoxyuridine and glyoxal-guanine under the following conditions, by using any one of the aforementioned three sets of enzyme solutions and the Bacillus stearothermophilus JTS 859 enzyme solution obtained in Production Example 2. Specifically, 5 mM phosphate buffer (pH 7) containing 30 mM 2′-deoxyuridine, 30 mM glyoxal-guanine, 100 mM boric acid, and 4 mM disodium ethylenediaminetetraacetate was first prepared. Each (2 mL) of the above four sets of enzyme solutions (which contain bacteria corresponding to 100 mL of the above culture solution) was added to 98 mL of the above phosphate buffer mixture, respectively, resulting in four sets of reaction mixtures. Each reaction mixture was kept at each reaction temperature for 48 hours, allowing the reaction to proceed. The results are shown in Table 3.
Workup
- customthe Bacillus stearothermophilus JTS 859 enzyme solution obtained in Production Example 2
- customwas first prepared
- customrespectively, resulting in four sets of reaction mixtures
- customthe reaction
Sources & evidence
- ORD record
- ord-8047a8aa0b204e6482842857d3b573ce
- Source dataset
- uspto-grants-2003_09
- Patent number
- US06620596B2
Linked notes
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