5'-Adp → 5'-Atp

HRID 1249663

Reaction equation

HRID 1249663 reaction equation
[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4][P:5]([OH:8])([OH:7])=[O:6])(=[O:3])[OH:2]>C(OP(O)(O)=O)[C@H]1O[C@H](OP(OP(O)(O)=O)(O)=O)[C@H](O)[C@@H]1O>[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4][P:5]([O:7][P:1]([OH:4])([OH:3])=[O:2])([OH:8])=[O:6])(=[O:2])[OH:3]

Participants

3

Reactant 1

HCID 60225'-Adp

Product 1

HCID 59575'-Atp

Reagents, catalysts & solvents 1

HCID 7339Phosphoribosyl PyrophosphateSolvent3×

Procedure

The following additions were made to each reaction: 2 μl of PRPP, 2 μl of PRPP Synthetase, and 20 μl PRPP Synthetase buffer. The reactions proceeded at 37° C. for 28 minutes at which time the reactions were transferred to 100 μl LAR containing 2 μl of ADP and 2 μl of NDPK. This second reaction was allowed to proceed at room temperature for 20 min. The amount of ATP produced was measured by the addition of 10 ng of Luciferase followed by measuring light output with a luminometer. The data is presented in Table 30.

Workup

  1. customThe following additions were made to each reaction
  2. customThis second reaction
  3. waitto proceed at room temperature for 20 min

Sources & evidence

ORD record
ord-e995be76fe084eddae8aedc9e9ed05a6
Source dataset
uspto-grants-2002_01
Patent number
US06335162B1

Linked notes

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