Phosphate → D-(-)-Fructose

Reaction equation

[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].P([O-])([O-])([O-])=O>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

Reactant 2

HCID 1061Phosphate

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×

Product 1

HCID 5984D-(-)-Fructose

Procedure

To a glucose isomerase solution are added a 0.1 M D-glucose solution, a 0.05 M phosphate buffer solution and a 0.005 M MgSO4.7H2O solution. While maintaining at pH 7.0, the reaction is continued at 70° C. for one hour and the amount of fructose produced is measured. The unit of activity is expressed in terms of "unit" and the amount of enzyme which produces 1 mg of fructose under the above conditions is defined as 1 unit.

Workup

temperatureWhile maintaining at pH 7.0

Sources & evidence

ORD record: ord-63cd645593ce42c19eeaa11c501d5463
Source dataset: uspto-grants-1981_03
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04255521

Reaction notes

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