D-1,4-Dithiothreitol → Octyl Maleimide

Reaction equation

C(S)[C@@H:2]([OH:7])[C@H:3](O)[CH2:4]S.[CH2:9]([C:17]1[C:18]([NH:20][C:21](=O)[CH:22]=1)=[O:19])[CH2:10][CH2:11][CH2:12][CH2:13]CCC>CS(C)=O>[CH2:21]([N:20]1[C:18](=[O:19])[CH:4]=[CH:3][C:2]1=[O:7])[CH2:22][CH2:17][CH2:9][CH2:10][CH2:11][CH2:12][CH3:13]

Participants

Procedure

For a vehicle control, combine a solution of vehicle (5 mM NaPO4 pH 5.5, 150 mM NaCl, 0.5 mM DTT) with PBS (Gibco product #20012-027, pH 7.2) in a 1:10 ratio. Add 1/400 vol. of 20 mM Octylmaleimide in DMSO and a 1/400 vol. of DMSO to give a final concentration of 50 μM N-octylmaleimide and 0.5% DMSO. Finally, add 1:1000 vol. of 0.35 M DTT.