N-Acetyl-DL-methionine → L-Methionine

HRID 127998

Reaction equation

HRID 127998 reaction equation
C([NH:4][CH:5]([C:10]([OH:12])=[O:11])[CH2:6][CH2:7][S:8][CH3:9])(=O)C>[Co](Cl)Cl>[NH2:4][C@H:5]([C:10]([OH:12])=[O:11])[CH2:6][CH2:7][S:8][CH3:9]

Participants

3

Reactant 1

HCID 6180N-Acetyl-DL-methionine

Product 1

HCID 6137L-Methionine

Reagents, catalysts & solvents 1

HCID 24288Cobalt(II) chlorideCatalyst

Conditions

Temperature
37 °C

Procedure

The enzymatic activity of the complex is determined by contacting 500 mg. of complex with 50 ml. of an 0.4 weight percent solution of N-acetyl-DL-methionine in 0.1 M TRIS buffer, of pH 8, containing 5·10-4M of cobalt chloride, at 37° C., and agitated for 30 minutes. The complex is filtered and the L-methionine formed is determined in the filtrate.

Workup

  1. filtrationThe complex is filtered

Sources & evidence

ORD record
ord-1d9ce5b55ed74958b0057f98b8ac68e6
Source dataset
uspto-grants-1981_03
Patent number
US04258133

Linked notes

Loading…