N-Acetyl-DL-methionine → L-Methionine
Reaction equation
C([NH:4][CH:5]([C:10]([OH:12])=[O:11])[CH2:6][CH2:7][S:8][CH3:9])(=O)C>[Co](Cl)Cl.O>[NH2:4][C@H:5]([C:10]([OH:12])=[O:11])[CH2:6][CH2:7][S:8][CH3:9]Participants
4
Reactant 1
Product 1
Reagents, catalysts & solvents 2
Conditions
- Temperature
- 37 °C
Procedure
The enzymatic activity of the acylase in solution, before and after fixing, is determined by adding 0.1 ml. of the enzyme solution to 5 ml. of an 0.4 weight percent solution of N-acetyl-DL-methionine in 0.1 M TRIS buffer, of pH 8, containing 5·10-4 mols of cobalt chloride. The mixture is maintained for 30 minutes at 37° C., whereupon the reaction is stopped by immersing the receptacle in boiling water for 3 minutes. After cooling, the L-methionine formed is determined.
Workup
- additionby adding 0.1 ml
- customfor 3 minutes
- temperatureAfter cooling
Sources & evidence
- ORD record
- ord-cebb56e106ae4cdaa34d3fb8f0a26a08
- Source dataset
- uspto-grants-1981_03
- Patent number
- US04258133
Reaction notes
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