Reaction equation
[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][C@H:14]([O:16][C@@H:17]([C@H:22]([OH:27])[C@@H:23]([OH:26])[CH:24]=[O:25])[C@H:18]([OH:21])[CH2:19][OH:20])[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]>C([O-])(=O)C>[O:8]=[CH:6][C@@H:5]([C@H:4]([C@@H:3]([C@@H:2]([CH2:1][OH:34])[OH:7])[OH:33])[OH:32])[OH:31].[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][C@H:14]([O:16][C@@H:17]([C@H:22]([OH:27])[C@@H:23]([OH:26])[CH:24]=[O:25])[C@H:18]([OH:21])[CH2:19][OH:20])[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]Participants
Reactant 1
Product 2
Reagents, catalysts & solvents 1
Procedure
The enzymatic activity of α-isomaltosyl-transferring enzyme can be assayed as follows: Dissolve panose in 100 mm acetate buffer (pH 6.0) to give a concentration of 2% (w/v) f or a substrate solution, add 0.5 ml of an enzyme solution to 0.5 ml of the substrate solution, and keep the mixture at 35° C. for 30 min to proceed the formation of cyclotetrasaccharide from panose. In the reaction system, glucose is formed together with cyclotetrasaccharide from panose. After the reaction, boil the reaction mixture for 10 min to suspend the reaction. Subject the resulting reaction mixture to the glucose oxidase method to quantify the glucose formed in the reaction mixture. In the present invention, one unit activity of α-isomaltosyl-transferring enzyme is defined as the enzyme amount that forms one micromole of glucose per minute under the above enzymatic reaction conditions.
Workup
- customto give a concentration of 2% (w/v) f
- customat 35° C.
- customfor 30 min
Sources & evidence
- ORD record
- ord-830cf9066441413db5f6eab4ca695e89
- Source dataset
- uspto-grants-2007_05
- Patent number
- US07223570B2
Linked notes
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