Pyridoxal hydrochloride → Pyridoxal

HRID 1354531

Reaction equation

HRID 1354531 reaction equation
CC([O-])=O.[Na+].[CH3:6][C:7]1[C:12]([OH:13])=[C:11]([CH:14]=[O:15])[C:10]([CH2:16][OH:17])=[CH:9][N:8]=1.Cl.[BH3-]C#N.[Na+]>O>[CH3:6][C:7]1[N:8]=[CH:9][C:10]([CH2:16][OH:17])=[C:11]([CH:14]=[O:15])[C:12]=1[OH:13]

Participants

5

Reactant 3

HCID 6171Pyridoxal hydrochlorideHCID 517045Sodium AcetateHCID 5003444Sodium cyanoborohydride

Product 1

HCID 1050Pyridoxal

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

PNA oligomer SEQ ID NO:63 or PNA oligomer SEQ ID NO:64 is treated according to the procedure of Example 62 to convert the N—PHTH group to the free amine. The resulting PNA oligomer is dissolved in 100 microliters of water, and 100 ml of 1M NaOAc buffer (pH 5.0) is added followed by 5 mg of pyridoxal hydrochloride (24 μmols) and 50 μl of 60 mM NaCNBH3 solution. The solution is vortexed, left overnight, and is then passed through a Sephadex G-25 column and further purified in an analytical HPLC column.

Workup

  1. additionPNA oligomer SEQ ID NO:63 or PNA oligomer SEQ ID NO:64 is treated
  2. customfurther purified in an analytical HPLC column

Sources & evidence

ORD record
ord-9822b87070304004a6400ec5607cd322
Source dataset
uspto-grants-2007_05
Patent number
US07223833B1

Linked notes

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