Carbonate Ion → Biotin

HRID 14353

Reaction equation

C1C(=O)N(OC(CCCCCN[C:17]([CH2:19][CH2:20][CH2:21][CH2:22][C@@H:23]2[S:27][CH2:26][C@@H:25]3[NH:28][C:29]([NH:31][C@H:24]23)=[O:30])=[O:18])=O)C(=O)C1.C(=O)([O-])[O-:33]>>[OH:33][C:17]([CH2:19][CH2:20][CH2:21][CH2:22][C@H:23]1[C@@H:24]2[C@@H:25]([NH:28][C:29]([NH:31]2)=[O:30])[CH2:26][S:27]1)=[O:18]

Participants

Reactant 2

HCID 19660Carbonate Ion

HCID 83874Nhs-LC-biotin2×

Product 1

HCID 171548Biotin

Procedure

Anti-rabbit IgG monoclonal antibodies (2B9 5 μg/mL) dissolved in 0.1 M carbonate buffer (pH 8.5) and NHS-LC-BIOTIN (PIERCE, 25 μg/mL) were mixed and stirred at room temperature for four hours using a stirrer. IgG and NHS-LC-BIOTIN were mixed at a mole ratio of 1:60. After stirring, this solution was dialyzed against physiological phosphate buffered saline (PBS) to obtain biotin-labeled antibodies.

Workup

stirringAfter stirring
customthis solution was dialyzed against physiological phosphate buffered saline (PBS)

Sources & evidence

ORD record: ord-94874c96d452428c957b90a24f0b4475
Source dataset: uspto-grants-2010_06
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07732154B2

Linked notes

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