L-(+)-Cysteine → HCID 86604979, compound

HRID 1593363

Reaction equation

HRID 1593363 reaction equation
O.O.O.O.O.O.[Cl-].[Cr+3:8].[Cl-].[Cl-].[NH2:11][C@H:12]([C:15]([OH:17])=[O:16])[CH2:13][SH:14].[P:18]([O:30][CH2:31][C@H:32]1[O:36][C@@H:35]([N:37]2[C:46]3[N:45]=[CH:44][N:43]=[C:41]([NH2:42])[C:40]=3[N:39]=[CH:38]2)[C@H:34]([OH:47])[C@@H:33]1[OH:48])([O:21][P:22]([O:25][P:26]([OH:29])([OH:28])=[O:27])([OH:24])=[O:23])(=[O:20])[OH:19]>O>[NH2:11][C@H:12]([C:15]([OH:17])=[O:16])[CH2:13][SH:14].[Cr:8].[P:18]([O:30][CH2:31][C@H:32]1[O:36][C@@H:35]([N:37]2[C:46]3[N:45]=[CH:44][N:43]=[C:41]([NH2:42])[C:40]=3[N:39]=[CH:38]2)[C@H:34]([OH:47])[C@@H:33]1[OH:48])([O:21][P:22]([O:25][P:26]([OH:28])([OH:29])=[O:27])([OH:24])=[O:23])(=[O:19])[OH:20]

Participants

5

Reactant 3

HCID 5862L-(+)-CysteineHCID 59575'-AtpHCID 166918Chromium(3+) trichloride hexahydrate

Product 1

HCID 86604979HCID 86604979, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Equimolar amounts of chromium chloride hexahydrate, cysteine, and ATP (10 mM each) were mixed in 50 ml of water at a pH of around 3. The mixture was then incubated at room temperature for 1-2 hours to form the chromium-cysteine-ATP complex. This complex was purified by loading 50 ml of the 10 mM chromium-cysteine-ATP mixture onto a SE53 ion-exchange column having a bed volume of approximately 30 ml. The loaded column was washed with 100 ml of water in order to wash off unbound materials. The bound chromium complex was then eluted with 0.25 M ammonium hydroxide. The eluted fractions (4 ml/fraction) were monitored with a UV spectrophotometer for absorption at wavelengths A260 and A280. An example of the eluting profile is shown in FIG. 1.

Workup

  1. customto form the chromium-cysteine-ATP complex
  2. customThis complex was purified
  3. washThe loaded column was washed with 100 ml of water in order
  4. washto wash off unbound materials
  5. washThe bound chromium complex was then eluted with 0.25 M ammonium hydroxide
  6. customThe eluted fractions (4 ml/fraction) were monitored with a UV spectrophotometer for absorption at wavelengths

Sources & evidence

ORD record
ord-067b413668c647c7b20800f34fc9febf
Source dataset
uspto-grants-2007_11
Patent number
US07297686B2

Linked notes

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