CC([S@@]([NH:7][C@:8]([C:23]1[CH:28]=[CH:27][C:26]([O:29][CH2:30][CH2:31][CH2:32][CH2:33][CH2:34][C:35]([F:38])([F:37])[F:36])=[CH:25][CH:24]=1)([CH2:13][C:14](=[O:22])[C:15]1[CH:20]=[CH:19][C:18]([CH3:21])=[CH:17][CH:16]=1)[C:9]([F:12])([F:11])[F:10])=O)(C)C.Cl>CO.O1CCOCC1>[NH2:7][C@@:8]([C:23]1[CH:28]=[CH:27][C:26]([O:29][CH2:30][CH2:31][CH2:32][CH2:33][CH2:34][C:35]([F:36])([F:37])[F:38])=[CH:25][CH:24]=1)([C:9]([F:12])([F:11])[F:10])[CH2:13][C:14]([C:15]1[CH:20]=[CH:19][C:18]([CH3:21])=[CH:17][CH:16]=1)=[O:22]To a solution of Intermediate 8D (482 mg, 0.852 mmol) in MeOH (4 mL) was added 4 M HCl (1 mL, 4.00 mmol) in dioxane. The resulting mixture was stirred at rt for 2 h and then concentrated. The residue was taken up in EtOAc, washed with saturated NaHCO3 and brine, dried (MgSO4), filtered and concentrated to afford Intermediate (386 mg, 58%) as a colorless oil, which was used for the subsequent reaction without further purification. LCMS Anal. Calc'd for C23H25F6NO2 461.18, found [M+H] 461.9.
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