FC(F)(F)C(O)=O.[O:8]=[C:9]([NH:42][C:43]1[NH:44][CH2:45][CH2:46][CH2:47][N:48]=1)[CH2:10][CH2:11][CH2:12][O:13][C:14]1[CH:41]=[CH:40][C:17]([CH2:18][C@@H:19]([C:33]([O:35]C(C)(C)C)=[O:34])[NH:20][C:21]2[N:25]([CH2:26][C:27]3[CH:32]=[CH:31][CH:30]=[CH:29][CH:28]=3)[N:24]=[N:23][N:22]=2)=[CH:16][CH:15]=1.C1(C)C=CC=CC=1>ClCCl>[O:8]=[C:9]([NH:42][C:43]1[NH:44][CH2:45][CH2:46][CH2:47][N:48]=1)[CH2:10][CH2:11][CH2:12][O:13][C:14]1[CH:41]=[CH:40][C:17]([CH2:18][C@@H:19]([C:33]([OH:35])=[O:34])[NH:20][C:21]2[N:25]([CH2:26][C:27]3[CH:32]=[CH:31][CH:30]=[CH:29][CH:28]=3)[N:24]=[N:23][N:22]=2)=[CH:16][CH:15]=12 ml of trifluoroacetic acid is added to 135 mg of ester 5-2 in 5 ml of dichloromethane, followed by agitating for 3 hours at ambient temperature, adding 10 ml of toluene, evaporating under reduced pressure, taking up in dichloromethane and crystallizing by adding an Et2O/pentane mixture. 92 mg of 5-3 is obtained.
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