Reaction equation
[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4].C1(=O)NC(=O)C=C1.P([O-])([O-])([O-])=O>>[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4]Participants
3
Procedure
To the Fab' (1 mg) prepared in the above process (a) was added the β-Gal-maleimide (3.9 mg) prepared in the process (b) and the mixture was reacted at 30° C. for 30 minutes. The reaction solution was centrifuged and the supernatant was subjected to Sepharose 6B column (φ1.5 cm×50 cm, eluent: 10 mM phosphate buffer, pH 6.5, containing 0.1% BSA) to give a β-Gal-Fab' fragment.
Workup
- customthe mixture was reacted at 30° C. for 30 minutes
Sources & evidence
- ORD record
- ord-46f60e02cd01433ea273c41e08592afb
- Source dataset
- uspto-grants-1995_01
- Patent number
- US05378608
Linked notes
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