Thiodiglycol → Deoxycholic Acid

HRID 1813888Notes

Reaction equation

HRID 1813888 reaction equation
C(O)CS[CH2:4][CH2:5][OH:6].CC(CC([C:16]1[CH:21]=[CH:20][C:19](OCCOCCO)=[CH:18][CH:17]=1)(C)C)(C)C.CCCCCCCCCCCC[O:41]CCO.[CH3:45][CH2:46][CH2:47][CH2:48][CH2:49][CH2:50][CH2:51][CH2:52][CH2:53][CH2:54][CH2:55][CH2:56][O:57][S:58]([O-:61])(=[O:60])=[O:59].[Na+:62].[CH3:63][C@@H:64]([C@@H:84]1[C@@:88]2(C)[C@@H:89]([OH:104])[CH2:90][C@@H:91]3[C@@:96]4([CH3:102])[CH2:97][CH2:98][C@@H](O)C[C@H:95]4C[C@@H](O)[C@H]3[C@@H:87]2CC1)[CH2:65][CH2:66][C:67](NCCC[N+](CC(O)CS([O-])(=O)=O)(C)C)=[O:68]>>[CH3:45][CH2:46][CH2:47][CH2:48][CH2:49][CH2:50][CH2:51][CH2:52][CH2:53][CH2:54][CH2:55][CH2:56][O:57][S:58]([O-:61])(=[O:60])=[O:59].[Na+:62].[CH3:63][C@@H:64]([C@@H:84]1[C@@:88]2([CH3:87])[C@@H:89]([OH:104])[CH2:90][C@@H:91]3[C@@:96]4([CH3:102])[CH2:97][CH2:98][C@@H:5]([OH:6])[CH2:4][C@H:95]4[CH2:18][CH2:17][C@H:16]3[C@@H:21]2[CH2:20][CH2:19]1)[CH2:65][CH2:66][C:67]([OH:68])=[O:41]

Participants

7

Reactant 5

HCID 5447ThiodiglycolHCID 247502-(Dodecyloxy)ethanolHCID 24775Poly(oxy-1,2-ethanediyl), alpha-((1,1,3,3-tetramethylbutyl)phenyl)-omega-hydroxy-, potassium salt (1:1)HCID 1221453-((3-Cholamidopropyl)dimethylammonio)-2-hydroxy-1-propanesulfonateHCID 3423265Sodium Lauryl Sulfate

Product 2

HCID 222528Deoxycholic AcidHCID 3423265Sodium Lauryl Sulfate

Procedure

The FIG. 7 shows the results. The samples were loaded on the gels in the following order (from left to right) lane-1 control sample, lane 2-3 thiodiglycol, lane 4-5 Tween-20, lane 6-7 NP-40, lane 8-9 Brij-35, lane 10 SDS, lane 11 CHAPSO, 12-13 Triton-X100, lane 14 CHAP lane 15 control. The electrophoresis results shows (after a comparison of several typical runs) that, with exception of SDS and de-oxycholate treated protein samples, most protein samples containing detergent showed batch to batch variation in protein precipitation yield. The protein precipitation yield depended on the techniques used for harvesting the precipitated protein. There were occasions when the precipitation yield of duplicate samples varied between 100% to 50%. Protein samples containing SDS invariably showed substantially 100% yield in multiple tests.

Sources & evidence

ORD record
ord-2619c2e4fef642bd912dcf54c1e6b415
Source dataset
uspto-grants-2005_04
Patent number
US06875617B2

Reaction notes

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