Hydrochloric Acid → (+)-Lactose

HRID 1831025

Reaction equation

HRID 1831025 reaction equation
Cl.[OH:2][C@@H:3]1[O:22][C@H:21]([CH2:23][OH:24])[C@@H:8]([O:9][C@@H:10]2[O:18][C@H:17]([CH2:19][OH:20])[C@H:15]([OH:16])[C@H:13]([OH:14])[C@H:11]2[OH:12])[C@H:6]([OH:7])[C@H:4]1[OH:5].P([O-])([O-])([O-])=O>O>[OH:2][CH:3]1[O:22][C@H:21]([CH2:23][OH:24])[C@@H:8]([O:9][C@@H:10]2[O:18][C@H:17]([CH2:19][OH:20])[C@H:15]([OH:16])[C@H:13]([OH:14])[C@H:11]2[OH:12])[C@H:6]([OH:7])[C@H:4]1[OH:5]

Participants

5

Reactant 3

HCID 313Hydrochloric AcidHCID 1061PhosphateHCID 6134beta-Lactose

Product 1

HCID 440995(+)-Lactose

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

A solution of 0.2 mg of bovine pancreatic insulin (Wako Pure Chemical Industries, Itd.) in 1 mL of a 2 mmol/L aqueous solution of hydrochloric acid, a solution of 320 mg of anhydrous β-lactose (Nacalai Tesque, Inc.) in 2 mL of distilled water, and 1 mL of a 200 mmol/L phosphate buffer (pH 8.4) were mixed in a test tube to give a mixture having a pH of about 8.1. The test tube was put into a constant temperature water bath at 40° C. to cause reaction. The reaction mixture was sampled after 0, 5 and 24 hours, and analyzed for products obtained by the reaction of insulin with lactose by high performance liquid chromatography (HPLC) under the following conditions.

Workup

  1. customto give a mixture
  2. customThe test tube was put into a constant temperature water bath at 40° C.
  3. customreaction
  4. aliquotThe reaction mixture was sampled after 0, 5 and 24 hours

Sources & evidence

ORD record
ord-879b1b08a00f46d492fa79bee6d9c096
Source dataset
uspto-grants-2006_06
Patent number
US07067618B1

Linked notes

Loading…