N-Acetyl-D-Glucosamine → D-Glucosamine
Reaction equation
Cl.N1C2C(=CC=CC=2)C=C1.C([NH:14][C@@H:15]1[C@@H:21]([OH:22])[C@H:20]([OH:23])[C@@H:19]([CH2:24][OH:25])[O:18][CH:16]1[OH:17])(=O)C>>[OH:17][CH:16]1[O:18][C@H:19]([CH2:24][OH:25])[C@@H:20]([OH:23])[C@H:21]([OH:22])[C@H:15]1[NH2:14]Participants
3
Procedure
An enzyme solution (0.1 ml) is added to 0.3 ml of 100 mM phosphate buffer (pH 7.8) containing 0.1 ml of 1% N-acetyl-D-glucosamine as a substrate, and the resultant mixture is incubated at 37° C. for 30 minutes. The amount of D-glucosamine formed is determined colorimetrically using indole-HCl according to the method of Z. Dische and E. Borenfreund [J. Biol. Chem., 184:517 (1950)]. One unit of the enzyme is defined as the amount of the enzyme which is capable of producing 1 μmol D-glucosamine from N-acetyl-D-glucosamine per minute at 37° C.
Sources & evidence
- ORD record
- ord-78ad018610204225840c56fed8dc6e9d
- Source dataset
- uspto-grants-1995_06