N-Acetyl-DL-methionine → L-Methionine

HRID 225200

Reaction equation

HRID 225200 reaction equation
C([NH:4][CH:5]([C:10]([OH:12])=[O:11])[CH2:6][CH2:7][S:8][CH3:9])(=O)C>P([O-])([O-])([O-])=O.[Co](Cl)Cl>[NH2:4][C@H:5]([C:10]([OH:12])=[O:11])[CH2:6][CH2:7][S:8][CH3:9]

Participants

4

Reactant 1

HCID 6180N-Acetyl-DL-methionine

Product 1

HCID 6137L-Methionine

Reagents, catalysts & solvents 2

HCID 24288Cobalt(II) chlorideCatalystHCID 1061PhosphateSolvent

Procedure

To test the bound aminoacylase activity, 100 mg of the charged carrier were carefully shaken for 2 minutes at room temperature in 3 ml of 0.1 molar phosphate buffer solution (pH 7.6) containing 10 mg/ml of N-acetyl-D,L-methionine and 0.36 mg of cobalt chloride (CoCl2 ·6 H2O). The amount of L-methionine formed was then found by reaction with L-aminoacid-oxidase and determination of the resulting hydrogen peroxide with peroxidase by UV-spectrophotometric measurement of o-dianisidine (in the oxidized, ie. quinoid, form) (Methods in Enzymology, Vol. II).

Sources & evidence

ORD record
ord-ae3e76166ad04b4d90e605eef355d101
Source dataset
uspto-grants-1980_11
Patent number
US04235973

Linked notes

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