NHS-Biotin → Biotin

HRID 22628

Reaction equation

HRID 22628 reaction equation
C1C(=O)N([O:8][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][CH:15]2[S:19][CH2:18][CH:17]3[NH:20][C:21]([NH:23][CH:16]23)=[O:22])=[O:10])C(=O)C1>C(=O)([O-])O.[Na+]>[OH:10][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][C@H:15]1[C@@H:16]2[C@@H:17]([NH:20][C:21]([NH:23]2)=[O:22])[CH2:18][S:19]1)=[O:8]

Participants

3

Reactant 1

HCID 434213NHS-Biotin

Product 1

HCID 171548Biotin

Reagents, catalysts & solvents 1

HCID 516892Sodium BicarbonateSolvent

Procedure

Each 3-mg portion of the 32-clone monoclonal antibodies prepared in (1) was mixed with 10 mg of biotinyl N-hydroxysuccinimide ester (product of Zymed) and the reaction was carried out in 0.1 M sodium hydrogen carbonate (pH 8) with stirring at room temperature for 3 hours. The reaction mixture was dialyzed overnight against 5 L of PBS at 4° C. to give the biotin-labeled monoclonal antibody.

Workup

  1. customThe reaction mixture was dialyzed overnight against 5 L of PBS at 4° C.

Sources & evidence

ORD record
ord-53d572a8e9d04798981f64c307b0a0f5
Source dataset
uspto-grants-2005_02
Patent number
US06849419B1

Linked notes

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