Nadh → Cotinine

HRID 260252

Reaction equation

[CH3:1][N:2]1[C:6](=[O:7])[CH2:5][CH2:4][C@H:3]1[C:8]1[CH:9]=[CH:10][CH:11]=[N:12][CH:13]=1.[CH2:14]([O:25][P:26]([OH:29])([OH:28])=[O:27])[C@H:15]1[O:20][C@@H:19]([OH:21])[C@H:18]([OH:22])[C@@H:17]([OH:23])[C@@H:16]1[OH:24].[CH:30]1[N:31]=[C:32]([NH2:73])[C:33]2[N:38]=[CH:37][N:36]([C@@H:39]3[O:43][C@H:42]([CH2:44][O:45][P:46]([O:49][P:50]([O:53][CH2:54][C@H:55]4[O:59][C@@H:58]([N:60]5[CH:65]=[C:64]([C:66]([NH2:68])=[O:67])[CH2:63][CH:62]=[CH:61]5)[C@H:57]([OH:69])[C@@H:56]4[OH:70])([OH:52])=[O:51])([OH:48])=[O:47])[C@@H:41]([OH:71])[C@H:40]3[OH:72])[C:34]=2[N:35]=1>>[CH3:1][N:2]1[C:6](=[O:7])[CH2:5][CH2:4][C@H:3]1[C:8]1[CH:9]=[CH:10][CH:11]=[N:12][CH:13]=1.[CH2:14]([O:25][P:26]([OH:29])([OH:28])=[O:27])[C@H:15]1[O:20][C@@H:19]([OH:21])[C@H:18]([OH:22])[C@@H:17]([OH:23])[C@@H:16]1[OH:24].[CH:30]1[N:31]=[C:32]([NH2:73])[C:33]2[N:38]=[CH:37][N:36]([C@@H:39]3[O:43][C@H:42]([CH2:44][O:45][P:46]([O:49][P:50]([O:53][CH2:54][C@H:55]4[O:59][C@@H:58]([N:60]5[CH:65]=[C:64]([C:66]([NH2:68])=[O:67])[CH2:63][CH:62]=[CH:61]5)[C@H:57]([OH:69])[C@@H:56]4[OH:70])([OH:52])=[O:51])([OH:48])=[O:47])[C@@H:41]([OH:71])[C@H:40]3[OH:72])[C:34]=2[N:35]=1

Participants

Reactant 3

HCID 439153Nadh

HCID 439427Beta-D-Glucose 6-phosphate

HCID 854019Cotinine

Product 2

HCID 854019Cotinine

HCID 131719436glucose-6-phosphate NAD

Procedure

Cotinine-enzyme conjugate was prepared at 1 mg/ml of protein and diluted 500-fold to a final concentration of 2.0 μg/ml. The anti-cotinine antibody was present in stock solution at an approximate concentration of 15 mg/ml. The glucose-6-phosphate-NAD solution was prepared by mixing 3 volumes of 0.11M glucose-6-phosphate with 2 volumes of 0.1M NAD.

Workup

additiondiluted 500-fold to a final concentration of 2.0 μg/ml

Sources & evidence

ORD record: ord-81ea2360901b4ab6ad533dc50ccdf0ca
Source dataset: uspto-grants-1996_06
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05527686

Linked notes

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