Nitrophenylgalactosides → Indole

HRID 263375

Reaction equation

HRID 263375 reaction equation
[CH:1]1[CH:6]=[C:5]([N+:7]([O-])=O)[C:4](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)=[CH:3][CH:2]=1.O[CH:23]1O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H:24]1O>>[NH:7]1[C:5]2[C:4](=[CH:3][CH:2]=[CH:1][CH:6]=2)[CH:24]=[CH:23]1

Participants

3

Reactant 2

HCID 96647Nitrophenylgalactosides2×HCID 440995(+)-Lactose

Product 1

HCID 798Indole

Procedure

Principle of Step 1: ONPG substituting for lactose is metabolized with β-galactosidase. At 44.5° C. E. coli grow and utilize ONPG and produce indole. β-galactoside turns the medium yellow. In the yellow colored medium, both fecal coliforms and E. coli are present. In samples which do not have a yellow color, there are no fecal coliforms and E. coli.

Workup

  1. customAt 44.5° C

Sources & evidence

ORD record
ord-eb280669c71847b59bf6ce4570e27668
Source dataset
uspto-grants-1997_07
Patent number
US05643743

Linked notes

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