MES NaOH → (2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol

HRID 276747

Reaction equation

HRID 276747 reaction equation
[C@@H:1]1([N:14]2[C:23]3[N:22]=[CH:21][N:20]=[C:18]([OH:19])[C:17]=3[N:16]=[CH:15]2)[O:13][C@H:6]([CH2:7][O:8]P(O)(O)=O)[C@@H:4]([OH:5])[C@H:2]1[OH:3].C1N(CCS(O)(=O)=O)CCOC1.[OH-].[Na+]>Cl>[C@@H:1]1([N:14]2[C:23]3[N:22]=[CH:21][N:20]=[C:18]([OH:19])[C:17]=3[N:16]=[CH:15]2)[O:13][C@H:6]([CH2:7][OH:8])[C@@H:4]([OH:5])[C@H:2]1[OH:3]

Participants

4

Reactant 2

HCID 23700530MES NaOHHCID 178527090HCID 178527090, compound2×

Product 1

HCID 178521066(2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol

Reagents, catalysts & solvents 1

HCID 313Hydrochloric AcidSolvent

Procedure

The phosphomonoesterase activity was measured under the following condition using 5′-inosinic acid as a substrate. The reaction was performed at 30° C. for 10 minutes in a reaction solution (1 ml) containing 10 μmol/ml of 5′-inosinic acid, 100 μmol/ml of MES/NaOH buffer (pH 6.0), and an enzyme. The reaction was stopped by adding 200 μl of 2 N hydrochloric acid. After that, precipitates were removed by centrifugation. Then, inosine produced by the hydrolytic reaction was quantitatively measured. An amount of enzyme to produce 1 μmol of inosine per 1 minute under this standard reaction condition was defined as 1 unit.

Workup

  1. customAfter that, precipitates
  2. customwere removed by centrifugation

Sources & evidence

ORD record
ord-376b0f99501d44c682fb5dd3c74cf564
Source dataset
uspto-grants-2002_03
Patent number
US06355472B2

Linked notes

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