Pyrophosphoric acid → HCID 178527090, compound

HRID 276748

Reaction equation

HRID 276748 reaction equation
[P:1]([O:5]P(O)(O)=O)([OH:4])([OH:3])=[O:2].[C@@H:10]1([N:19]2[C:28]3[N:27]=[CH:26][N:25]=[C:23]([OH:24])[C:22]=3[N:21]=[CH:20]2)[O:18][C@H:15]([CH2:16]O)[C@@H:13]([OH:14])[C@H:11]1[OH:12]>C([O-])(=O)C>[C@@H:10]1([N:19]2[C:28]3[N:27]=[CH:26][N:25]=[C:23]([OH:24])[C:22]=3[N:21]=[CH:20]2)[O:18][C@H:15]([CH2:16][O:5][P:1]([OH:3])([OH:4])=[O:2])[C@@H:13]([OH:14])[C@H:11]1[OH:12]

Participants

4

Reactant 2

HCID 1023Pyrophosphoric acidHCID 178521066(2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol

Product 1

HCID 178527090HCID 178527090, compound

Reagents, catalysts & solvents 1

HCID 175AcetateSolvent

Procedure

Pyrophosphoric acid (15 g/dl) and 8 g/dl of inosine were dissolved in an acetate buffer (pH 4.0). To the solution was added E. coli JM109 strain having introduced therein the above-mentioned mutant and wild-type acid phosphatase genes such that the concentration reached 200 mg/dl in terms of the dry cell weight. The reaction was conducted at 35° C. for 32 hours while maintaining the pH at 4.0, and the amount of 5′-inosinic acid formed over the course of time was measured. Inosinic acid formed was only 5′-inosinic acid, and the formation of 2′-inosinic acid and 3′-inosinic acid as by-products was not observed at all. The results are shown in FIG. 8.

Workup

  1. additionTo the solution was added E
  2. additionstrain having introduced
  3. concentrationthe above-mentioned mutant and wild-type acid phosphatase genes such that the concentration
  4. temperaturewhile maintaining the pH at 4.0

Sources & evidence

ORD record
ord-eb9c4c079f734c0a8a636d1a0d000670
Source dataset
uspto-grants-2002_03
Patent number
US06355472B2

Linked notes

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