Sucrose → Spectinomycin

HRID 336029

Reaction equation

C(O)[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.C1(CC(O)=O)C2C(=CC=CC=2)C=CC=1.C(NC1N=C2C(NC=N2)=CN=1)C1C=CC=CC=1.[CH3:55][C@H:56]1[O:62][C@H:61]2[O:63][C@H:64]3[C@H:69]([O:70][C@@:60]2([OH:77])[C:58](=[O:59])[CH2:57]1)[C@@H:68]([NH:71][CH3:72])[C@@H:67]([OH:73])[C@@H:66]([NH:74][CH3:75])[C@@H:65]3[OH:76].Cl.Cl>>[CH3:55][C@H:56]1[O:62][C@H:61]2[O:63][C@H:64]3[C@H:69]([O:70][C@@:60]2([OH:77])[C:58](=[O:59])[CH2:57]1)[C@@H:68]([NH:71][CH3:72])[C@@H:67]([OH:73])[C@@H:66]([NH:74][CH3:75])[C@@H:65]3[OH:76]

Participants

Reactant 4

HCID 5988Sucrose

HCID 68621-Naphthaleneacetic Acid

HCID 64740Spectinomycin Dihydrochloride

HCID 12922494N-Benzyl-7H-purin-2-amine

Product 1

HCID 15541Spectinomycin

Procedure

When calli are about 0.5 mm in size they are transferred to callus growth medium SH2 (Schenk & Hildebrandt, Can. J. Bot. 50:199-204 (1972)) with 30 g/l saccharose, 5 g/l agarose, 0.1 mg/l 1-naphtalene acetic acid (NAA) and 0.1 mg/l benzylaminopurin (BAP), and the selecting agent spectinomycin dihydrochloride at concentrations of 0-1000 mg/l. It was found that the optimal concentration of selection is 500 mg/l. The non-resistant calli appear as white calli. They also grow slower as compared to the control calli. The culture conditions are as described above for the above protoplast calli.

Workup

concentrationIt was found that the optimal concentration of selection

Sources & evidence

ORD record: ord-252de01952f54d03bdc6ddb409cdb7fa
Source dataset: uspto-grants-2014_11
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US08884099B2

Linked notes

Loading…