Hydrochloric Acid → Trehalose

HRID 339478

Reaction equation

HRID 339478 reaction equation
[CH2:1]([OH:23])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[O:14][C@H:13]([CH2:15][OH:16])[C@@H:12]([OH:17])[C@H:11]([OH:18])[C@H:10]2[OH:19])[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22].O.O.Cl>O>[CH2:15]([OH:16])[C@H:13]1[O:14][C@H:9]([O:8][C@H:6]2[O:7][C@H:2]([CH2:1][OH:23])[C@@H:3]([OH:22])[C@H:4]([OH:21])[C@H:5]2[OH:20])[C@H:10]([OH:19])[C@@H:11]([OH:18])[C@@H:12]1[OH:17]

Participants

5

Reactant 3

HCID 313Hydrochloric AcidHCID 181978Trehalose, dihydrateHCID 178435178HCID 178435178, compound

Product 1

HCID 7427Trehalose

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Procedure

25.48 g of trehalose dihydrate was carefully weighed and transferred into a sterile polypropylene bottle, to which 350 ml of purified water was added at room temperature and stirred slowly until a clear solution was obtained. To the clear solution, 0.1 N HCl was added drop by drop to adjust the pH to 3.9, then the volume was adjusted with purified water to obtain 400 ml final volume. The pH was measured and found to be 4.2. The solution was filtered through a 0.22-micron filter and was used directly to dilute the protein solution.

Workup

  1. additionwas added at room temperature
  2. customwas obtained
  3. customto obtain 400 ml final volume
  4. filtrationThe solution was filtered through a 0.22-micron
  5. filtrationfilter
  6. additionto dilute the protein solution

Sources & evidence

ORD record
ord-f9c6540bbb214e2bbd626ed87959ca81
Source dataset
uspto-grants-2014_11
Patent number
US08895506B2

Linked notes

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