Sodium Chloride → Phosphate Buffered Saline

HRID 4161

Reaction equation

HRID 4161 reaction equation
[P:1]([O-:5])([O-:4])([O-:3])=[O:2].[K+:6].[K+].[K+].[P:9]([O-:13])([O-:12])([O-:11])=[O:10].[Na+:14].[Na+].[Na+].[Cl-:17].[Na+]>O>[OH:3][P:1]([O-:5])([OH:4])=[O:2].[OH:11][P:9]([O-:13])([O-:12])=[O:10].[Na+:14].[Na+:14].[Na+:14].[Cl-:17].[Cl-:17].[K+:6].[K+:6]

Participants

5

Reactant 3

HCID 5234Sodium Chloride2×HCID 24243Trisodium PhosphateHCID 62657Potassium Phosphate, Tribasic

Product 1

HCID 24978514Phosphate Buffered Saline

Reagents, catalysts & solvents 1

HCID 962WaterSolvent3×

Procedure

Phosphate buffered saline solution was prepared in the following manner. An 18.8 g. portion of anhydrous dibasic potassium phosphate was dissolved in 140 ml. distilled water preheated to 45° C. To this solution were added 3.3 g. monobasic sodium phosphate, and it was stirred until dissolved. Then 45 g. sodium chloride were added with stirring. After the sodium chloride was dissolved, the solution was brought to 200 ml. with distilled water. A 40 ml. portion of the above solution was then mixed with 960 ml. distilled water to form a 0.01M phosphate buffered saline solution (PBS) at pH 7.5. It was then stored at 4° C. Larger quantities were also prepared using the above proportions.

Workup

  1. customPhosphate buffered saline solution was prepared in the following manner
  2. custompreheated to 45° C
  3. additionTo this solution were added 3.3 g
  4. dissolutionuntil dissolved
  5. additionportion of the above solution was then mixed with 960 ml

Sources & evidence

ORD record
ord-e788ab5adecf4542b44f03394b9b1d4f
Source dataset
uspto-grants-1988_02
Patent number
US04724203

Linked notes

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