beta-Maltose → alpha-Maltotriose

HRID 460742

Reaction equation

HRID 460742 reaction equation
[CH2:1]([OH:23])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@H:12]([OH:17])[O:11][C@@H:10]2[CH2:18][OH:19])[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22].[O:24]=[CH:25][C@@H:26]([C@H:28]([C@@H:30]([C@@H:32]([CH2:34][OH:35])[OH:33])O)[OH:29])[OH:27]>NCC(O)=O>[CH2:1]([OH:23])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@@H:12]([O:17][C@H:30]3[C@H:28]([OH:29])[C@@H:26]([OH:27])[C@@H:25]([OH:24])[O:33][C@@H:32]3[CH2:34][OH:35])[O:11][C@@H:10]2[CH2:18][OH:19])[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22]

Participants

4

Reactant 2

HCID 6255beta-MaltoseHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 192826alpha-Maltotriose

Reagents, catalysts & solvents 1

HCID 750GlycineSolvent2×

Procedure

A portion of the amylase produced in Example I containing 90,000 amylase units/ml. was diluted 1/500 with 0.05 molar glycine buffer solution, and 80 ml. of the resulting solution were added to 250 ml. of an aqueous solution containing 0.0909 percent (weight/volume basis) amylose in 0.05 molar glycine buffer adjusted to pH 9.2 at 50° C. After 100 hours at 50° C. there was an apparent conversion of 75.5 percent of the amylose to maltose and a conversion of 4.6 percent of the amylose to glucose. A small quantity of maltotriose was also produced. Such analyses were carried out by well known procedures.

Workup

  1. customA portion of the amylase produced in Example I
  2. additioncontaining 90,000 amylase units/ml
  3. additionof the resulting solution were added to 250 ml
  4. customadjusted to pH 9.2 at 50° C

Sources & evidence

ORD record
ord-21b823d6b4914ff2b690e7e49282129b
Source dataset
uspto-grants-1977_12
Patent number
US04061541

Linked notes

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