Nadide (JAN/USAN/INN) → NADPH

HRID 483769

Reaction equation

[CH:1]1[N:2]=[C:3]([NH2:44])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([O:20][P:21]([O:24][CH2:25][C@H:26]4[O:30][C@@H:29]([N:31]5[CH:36]=[C:35]([C:37]([NH2:39])=[O:38])[CH2:34][CH:33]=[CH:32]5)[C@H:28]([OH:40])[C@@H:27]4[OH:41])([OH:23])=[O:22])([OH:19])=[O:18])[C@@H:12]([OH:42])[C@H:11]3[OH:43])[C:5]=2[N:6]=1.[CH:45]1[CH:50]=[N+:49]([C@@H:51]2[O:55][C@H:54]([CH2:56][O:57][P:58]([O:61][P:62]([O:65][CH2:66][C@H:67]3[O:71][C@@H:70]([N:72]4[C:76]5[N:77]=[CH:78][N:79]=[C:80]([NH2:81])[C:75]=5[N:74]=[CH:73]4)[C@H:69]([OH:82])[C@@H:68]3[OH:83])([OH:64])=[O:63])([OH:60])=[O:59])[C@@H:53]([OH:84])[C@H:52]2[OH:85])[CH:48]=[C:47]([C:86]([NH2:88])=[O:87])[CH:46]=1>>[CH:1]1[N:2]=[C:3]([NH2:44])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([O:20][P:21]([O:24][CH2:25][C@H:26]4[O:30][C@@H:29]([N:31]5[CH:36]=[C:35]([C:37]([NH2:39])=[O:38])[CH2:34][CH:33]=[CH:32]5)[C@H:28]([OH:40])[C@@H:27]4[OH:41])([OH:23])=[O:22])([OH:19])=[O:18])[C@@H:12]([OH:42])[C@H:11]3[O:43][P:58]([OH:60])([OH:59])=[O:57])[C:5]=2[N:6]=1.[CH:45]1[CH:50]=[N+:49]([C@@H:51]2[O:55][C@H:54]([CH2:56][O:57][P:58]([O:61][P:62]([O:65][CH2:66][C@H:67]3[O:71][C@@H:70]([N:72]4[C:76]5[N:77]=[CH:78][N:79]=[C:80]([NH2:81])[C:75]=5[N:74]=[CH:73]4)[C@H:69]([O:82][P:17]([OH:19])([OH:18])=[O:16])[C@@H:68]3[OH:83])([OH:64])=[O:63])([OH:60])=[O:59])[C@@H:53]([OH:84])[C@H:52]2[OH:85])[CH:48]=[C:47]([C:86]([NH2:88])=[O:87])[CH:46]=1

Participants

Reactant 2

HCID 5893Nadide (JAN/USAN/INN)

HCID 439153Nadh

Product 2

HCID 5884NADPH

HCID 5886NADP nicotinamide-adenine-dinucleotide phosphate

Conditions

Temperature: 100 °C

Procedure

To 0.9 ml of each of the above substrate solutions containing NADH or NAD+ was added 0.1 ml of 23 U/ml of the present enzyme, and the reaction was carried out at 30° C. for 20 minutes. Then, the reaction was terminated by heat treatment at 100° C. for 2 minutes, and the denatured protein was removed, after which the amount of NADPH or NADP+ produced by the reaction of the corresponding substrate was measured as follows. As a control solution, there was used a mixture of each substrate solution and the present enzyme in which the reaction had been terminated immediately after mixing them.

Workup

customThen, the reaction was terminated
customthe denatured protein was removed

Sources & evidence

ORD record: ord-780ec6230df3424eb00f2854834671cc
Source dataset: uspto-grants-1993_07
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05227299

Linked notes

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