Reaction equation
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.S([O-])([O-])(=O)=O.[NH4+].[NH4+].[K].S([O-])([O-])(=O)=O.[Mg+2].CC1[N+](CC2C=NC(C)=NC=2N)=CSC=1CCO.Cl.[Cl-].[OH:47][C:48](CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:49].[C:63]([NH2:71])(=O)[C:64]1[CH:69]=[CH:68][CH:67]=[N:66]C=1.NC(C(O)=O)C>>[NH2:71][C@H:63]([C:48]([OH:49])=[O:47])[CH2:64][CH2:69][CH2:68][CH2:67][NH2:66]Participants
Procedure
An aqueous solution medium having a composition of 10% of glucose, 2% of ammonium sulfate, 0.1% of potassium primary phosphate, 0.04% of magnesium sulfate, 0.001% of ferrous sulfate, 200 μg/l of thiamine hydrochloride, 500 μg/l of biotin, 5 ml/dl of Aji-Eki®, 1 mg/dl of nicotinamide and 0.1% of DL-alanine, at pH 7.0, was charged in an amount of 300 ml into separate small sized glass jar fermenters. After sterilizing in a conventional manner, the various L-lysine-producing bacterial strains shown in Table 11, which had been previously grown in bouillon slants at 30° C. for 48 hours, were inoculated thereon. Then, incubation was carried out at 31.5° C. for 48 hours at 1200 rpm at an aeration rate of 1/2 volume per minute, while keeping the pH at 7.0 by the addition of ammonia gas. After completion of the fermentation, the yield of L-lysine produced and accumulated in the solution based on glucose was determined and is shown in Table 11.
Workup
- additionat pH 7.0, was charged in an amount of 300 ml
- custominto separate small sized glass jar fermenters
Sources & evidence
- ORD record
- ord-b0238f302d32451580ab037873b18d0f
- Source dataset
- uspto-grants-1994_03
- Patent number
- US05294547
Linked notes
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