Czen 002 → Epinephrine

HRID 495894

Reaction equation

HRID 495894 reaction equation
CC([C@H:4]([NH:8][C:9]([C@H]1N(C([C@@H](NC(CNC([C@@H](NC([C@@H](NC([C@@H](NC(C(NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC(C)=O)CO)=O)CC2C=CC(O)=CC=2)=O)CO)=O)CCSC)=O)CCC(O)=O)=O)CC2NC=NC=2)=O)CC2C=CC=CC=2)=O)CCCNC(N)=N)=O)CC2C3C(=CC=CC=3)NC=2)=O)=O)CCCCN)=O)CCC1)=O)C(N)=O)C.O=[C:120]1[O:126][C@H:125]([C@H:127]([CH2:129][OH:130])O)[C:123](O)=[C:121]1[OH:122].[O:131]=[C:132]1[O:138][C@H:137]([C@H:139]([CH2:141][OH:142])[OH:140])[C:135]([O-:136])=[C:133]1[OH:134].[Na+:143]>>[CH3:4][NH:8][CH2:9][C@H:129]([OH:130])[C:127]1[CH:125]=[CH:123][C:121]([OH:122])=[C:120]([OH:126])[CH:132]=1.[O:131]=[C:132]1[O:138][C@H:137]([C@H:139]([CH2:141][OH:142])[OH:140])[C:135]([O-:136])=[C:133]1[OH:134].[Na+:143]

Participants

5

Reactant 3

HCID 16129664Czen 0022×HCID 23667548Sodium AscorbateHCID 54670067L-Ascorbic Acid

Product 2

HCID 5816EpinephrineHCID 23667548Sodium Ascorbate

Procedure

In a specific non-limiting embodiment of the invention Hu74 Medium is prepared as follows. Glutamine is added to Ham's F12 (GIBCO, Carlsbad, Calif.) at a concentration of 2 mM. Gentamicin is added to the culture medium to obtain a final concentration of 50 μg/ml. Fetal bovine serum (FBS, GIBCO, Carlsbad, Calif.) is added to the medium to obtain a 10% concentration (volume/volume). The medium containing FBS and gentamicin is stored at 4° C. and prepared fresh every two weeks. bFGF (PeproTech, Rocky Hill, N.J.) is dissolved in F12 medium to 2,500 ng/ml and stored in small vials at −70° C. The stored solution is added to the culture medium to obtain a final concentration of 25 ng/ml once a week. HGF (PeproTech, Rocky Hill, N.J.) is dissolved in F12 medium to 10,000 ng/ml and stored in small vials at −70° C. The stored solution is added to the culture medium to obtain a final concentration of 100 ng/ml once a week. An α-MSH solution is obtained by dissolving the α-MSH powder (Sigma, St. Louis, Mo.) to 100 μg/ml and is stored at −70° C. The stored solution of α-MSH is added to the culture medium to obtain a final concentration of 100 ng/ml once a week. (L)-Epinephrine bitartrate (Sigma, St. Louis, Mo.) is dissolved in PBS at 0.15 mg/ml with sodium ascorbate (Sigma, St. Louis, Mo.) (0.5 mg/ml) and stored at −70° C. Ephinedrine and ascorbic acid are added to the culture medium to obtain a final concentration of 1.5 μg/ml of epinephrine and 5 μg/ml sodium ascorbate once a week.

Workup

  1. customis stored at −70° C
  2. customto obtain a final concentration of 100 ng/ml once a week
  3. customstored at −70° C

Sources & evidence

ORD record
ord-d8a997f00b214d39a78232020d45c2f2
Source dataset
uspto-grants-2005_09
Patent number
US06943024B2

Linked notes

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