Ethylenediaminetetraacetic Acid → L-Cysteinylglycine

HRID 511885

Reaction equation

HRID 511885 reaction equation
C1[C:6]([S:7]SC2C=CC([N+]([O-])=O)=C(C(O)=O)C=2)=CC(C(O)=O)=C([N+]([O-])=O)C=1.[CH2:27]([N:38](CC(O)=O)CC(O)=O)[CH2:28][N:29](CC(O)=O)[CH2:30][C:31]([OH:33])=[O:32].P([O-])([O-])([O-])=[O:48].[K+].[K+].[K+]>>[CH2:6]([SH:7])[C@H:27]([NH2:38])[C:28]([NH:29][CH2:30][C:31]([OH:33])=[O:32])=[O:48]

Participants

4

Reactant 3

HCID 6049Ethylenediaminetetraacetic AcidHCID 62545,5'-Dithiobis(2-nitrobenzoic Acid)HCID 62657Potassium Phosphate, Tribasic

Product 1

HCID 439498L-Cysteinylglycine

Procedure

DTNB (5,5′-Dithio-bis-(2-nitrobenzoic acid; MM=396.36 g/mol) was prepared at 3.9 mg/ml (equivalent to 10 mM) in potassium phosphate buffer 50 mM, pH=7.5+0.1 mM EDTA. The reagent was used at 0.5 mM final concentration.

Workup

  1. concentrationconcentration

Sources & evidence

ORD record
ord-a254a0236c004c668a1605c274e29f40
Source dataset
uspto-grants-2015_08
Patent number
US09101783B2

Linked notes

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