Sodium Hydroxide → L-Leucine

HRID 638527

Reaction equation

HRID 638527 reaction equation
C([NH:4][C@H:5]([C:10]([OH:12])=[O:11])[CH2:6][CH:7]([CH3:9])[CH3:8])(=O)C.C(N[C@H](C(O)=O)[C@H](CC)C)(=O)C.[OH-].[Na+]>O>[NH2:4][C@H:5]([C:10]([OH:12])=[O:11])[CH2:6][CH:7]([CH3:9])[CH3:8]

Participants

5

Reactant 3

HCID 14798Sodium HydroxideHCID 70912N-Acetyl-L-leucineHCID 7036275N-Acetylisoleucine

Product 1

HCID 6106L-Leucine

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Conditions

Temperature
36 °C

Procedure

86.6 grams of this mixture of equal parts of N-acetyl-leucine and N-acetyl-isoleucine were suspended in 400 ml of water and adjusted to pH 7.0 with 50% aqueous sodium hydroxide. There were added 50 mg of CoCl2X6H2O and 50 mg of commerical pig kidney acylase (1200 U/mg) and the mixture was filled up to 500 ml with water. The clear solution was stirred at 36° C. for 6 hours. By filtering with suction, washing and drying there were obtained 17.9 grams of pure L-leucine having a rotary value of [α]D25 =+15.9°.

Workup

  1. additionThere were added 50 mg of CoCl2X6H2O and 50 mg of commerical pig kidney acylase (1200 U/mg)
  2. filtrationBy filtering with suction
  3. washwashing
  4. customdrying there

Sources & evidence

ORD record
ord-1d3a85980dba4ee1a0ce9052fe130086
Source dataset
uspto-grants-1985_12
Patent number
US04562153

Linked notes

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